CAT试剂盒，用于样本中过氧化氢酶活性分析，CAT Acti

Micro Catalase (CAT) Activity Assay Kit

产品概述

Micro Catalase (CAT) Activity Assay Kit（货号：KTB1040-E）是一种基于比色法的高灵敏度检测试剂盒，专为定量分析多种生物样本（包括血清、血浆、组织/细胞裂解液及其他生物液体）中的过氧化氢酶（CAT）活性而设计。本试剂盒通过直接测定CAT酶的过氧化功能，有效避免其他过氧化物酶的干扰，确保检测结果准确可靠。

主要特点与优势

• 广泛适用性：适用于多种样本类型，包括细胞裂解物、血清及血浆等。
• 高特异性：利用CAT特有的过氧化功能，显著降低非特异性反应。
• 宽检测范围：可检测2–75 µM范围内的CAT活性，灵敏度低至2 U/mL。
• 操作便捷：试剂盒提供完整组分，优化反应体系，适合高通量筛选。

检测原理

过氧化氢酶（CAT，EC 1.11.1.6）在生物体内催化过氧化氢（H₂O₂）分解为水和氧气，是细胞抗氧化系统的关键酶之一。本试剂盒利用CAT的过氧化活性，以甲醛为标准物，通过比色法在特定波长下测定产物含量，从而准确反映CAT酶活性水平。

试剂盒组分

• Assay Buffer (10×)
• Sample Diluent (10×)
• Lysis Buffer (10×)
• Methanol
• Formaldehyde standard (4.25 M)
• Catalase (control)
• Potassium Hydroxide
• Hydrogen Peroxide
• Chromogen
• Potassium Periodate

保存与运输条件

试剂盒应在接收后避光保存于-20℃。各组分具体存储条件请参见产品说明书。自发货日起，在推荐保存条件下有效期6个月。运输采用冰袋（蓝冰）保障试剂稳定性。

注意事项

• 如不立即使用，样本可存于-80℃，避免反复冻融。
• 过热可能导致CAT失活，故样本处理及检测过程需保持低温环境。
• CAT在高稀释状态下稳定性较差，建议样本浓缩并在稀释后30分钟内完成测定。

应用背景

过氧化氢酶广泛存在于好氧细胞中，在清除H₂O₂、减轻氧化应激损伤方面发挥关键作用。其活性与多种疾病机制相关，包括衰老、代谢紊乱及神经退行性疾病等。本试剂盒为氧化应激研究、药物筛选及细胞代谢分析提供有力工具。

产品规格与价格

规格	价格（元）	货期
96 T/96 S	316	现货（当天发货）
480 T/480 S	916	现货（次日发货）

警告

本产品仅限研究使用，不适用于人类或临床诊断。使用者应确保应用方式不违反相关专利或许可协议。对于因使用本产品可能导致的专利侵权或其他违规行为，供应商不承担任何责任。

别名：过氧化氢酶；CAT活性分析试剂盒；Catalase Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Catalase (CAT) Activity Assay Kit

Product Overview

The Micro Catalase (CAT) Activity Assay Kit (Product No.: KTB1040-E) is a highly sensitive colorimetric assay kit designed for the quantitative analysis of catalase (CAT) activity in various biological samples, including serum, plasma, tissue/cell lysates, and other biological fluids. By directly measuring the peroxidative function of CAT enzyme, this kit effectively avoids interference from other peroxidases, ensuring accurate and reliable detection results.

Key Features and Advantages

• Broad Applicability: Suitable for various sample types, including cell lysates, serum, and plasma.
• High Specificity: Utilizes the unique peroxidative function of CAT to significantly reduce non-specific reactions.
• Wide Detection Range: Capable of detecting CAT activity in the range of 2–75 µM, with sensitivity as low as 2 U/mL.
• User-Friendly Operation: The kit provides complete components and an optimized reaction system, making it suitable for high-throughput screening.

Assay Principle

Catalase (CAT, EC 1.11.1.6) catalyzes the decomposition of hydrogen peroxide (H₂O₂) into water and oxygen in living organisms, serving as a key enzyme in the cellular antioxidant system. This kit leverages the peroxidative activity of CAT, using formaldehyde as a standard, and employs colorimetry to measure product content at a specific wavelength, thereby accurately reflecting CAT enzyme activity levels.

Kit Components

• Assay Buffer (10×)
• Sample Diluent (10×)
• Lysis Buffer (10×)
• Methanol
• Formaldehyde standard (4.25 M)
• Catalase (control)
• Potassium Hydroxide
• Hydrogen Peroxide
• Chromogen
• Potassium Periodate

Storage and Shipping Conditions

The kit should be stored protected from light at -20℃ upon receipt. Refer to the product manual for specific storage conditions of each component. The shelf life is 6 months from the date of shipment when stored under recommended conditions. Shipping is conducted with ice packs (blue ice) to ensure reagent stability.

Precautions

• If not used immediately, samples can be stored at -80℃ to avoid repeated freeze-thaw cycles.
• Excessive heat may cause CAT inactivation; therefore, sample handling and detection processes should be performed in a low-temperature environment.
• CAT is less stable under high dilution conditions; it is recommended to concentrate samples and complete the assay within 30 minutes after dilution.

Application Background

Catalase is widely present in aerobic cells and plays a critical role in clearing H₂O₂ and mitigating oxidative stress damage. Its activity is associated with various disease mechanisms, including aging, metabolic disorders, and neurodegenerative diseases. This kit provides a powerful tool for oxidative stress research, drug screening, and cellular metabolism analysis.

Specifications and Pricing

Specification	Price (¥)	Lead Time
96 T/96 S	316	In stock (shipped same day)
480 T/480 S	916	In stock (shipped next day)

Warning

This product is for research use only and is not intended for human or clinical diagnostic use. Users should ensure that their application does not violate relevant patents or licensing agreements. The supplier assumes no liability for any patent infringement or other violations that may arise from the use of this product.

Alias: Catalase; CAT Activity Assay Kit; Catalase Activity Assay Kit

Note: The above information may be subject to change due to product optimization and upgrades. For specific details, please consult Yacoin Biotechnology.