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- Bioss
- bs-13496R-HRP
- 2025年12月01日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bs-13496R-HRP |
| 英文名称 | GPAA1 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的糖基磷脂酰肌醇锚连接蛋白1抗体 |
| 英文别名 | Anchor attachment protein 1(Gaa1p, yeast) homolog; GAA1; GAA1; GAA1; GAA1 protein homolog; Glycophosphatidylinositol anchor attachment 1; Glycosylphosphatidylinositol anchor attachment 1 protein; Glycosylphosphatidylinositol anchor attachment 1 protein; Glycosylphosphatidylinositol anchor attachment 1 protein; Glycosylphosphatidylinositol anchor attachment protein 1 homolog(yeast); GPAA 1; GPAA1; GPAA1_HUMAN; GPAA1P anchor attachment protein 1 homolog; GPI anchor attachment protein 1; GPI anchor attachment protein 1; GPI anchor attachment protein 1; GPI transamidase subunit; hGAA1. |
| 产品应用 | ELISA=1:500-5000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GPAA1 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Forms a complex with PIGK/GPI8, PIGT, PIGU and PIGS. |
| 亚细胞定位 | Endoplasmic reticulum membrane. |
| 组织特异性 | Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues. |
| 功能 | Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | Glycosylphosphatidylinositol (GPI) acts as a membrane anchor for cell surface proteins. Glycosylphosphatidylinositol anchor attachment 1 protein (GPAA1), also designated GPI anchor attachment protein 1 or GAA1 protein homolog, is a membrane protein localized to the endoplasmic reticulum which is involved in GPI-anchor biosynthesis. GPAA1 is crucial for GPI-anchoring of precursor proteins and catalyzes the attachment of GPI to proteins containing a C-terminal GPR attachment signal. GAA1 contains an N-terminal signal sequence, one cAMP- and cGMP-dependent protein kinase phosphorylation site, two potential N-glycosylation sites, one leucine zipper pattern and eight putative transmembrane domains. GPAA1 is ubiquitously expressed and shows higher levels of expression in fetal tissues than in adult tissues. |
| 应用 | 推荐稀释比例 |
| {ELISA} | {1:500-5000} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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