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- bsm-43009M-PerCP-Cy5.5
- 2025年12月01日
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100ul
| 产品编号 | bsm-43009M-PerCP-Cy5.5 |
| 英文名称 | TIMP1 Mouse mAb, PerCP-Cy5.5 conjugated |
| 中文名称 | PerCP-Cy5.5标记的金属蛋白酶组织抑制因子-1单克隆抗体 |
| 英文别名 | Clgi; Collagenase inhibitor; EPA; EPO; Erythroid Potentiating Activity; Fibroblast collagenase inhibitor; FLJ90373; HC; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP 1; TIMP; TIMP metallopeptidase inhibitor 1; TIMP1 protein; Tissue Inhibitor of Metalloproteinase 1; Tissue inhibitor of metalloproteinases; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor. |
| 产品应用 | IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human TIMP1 protein |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > Angiogenesis > ECM enzymes > TIMPs Cancer > Invasion/microenvironment > ECM > Extracellular matrix > TIMPs Cardiovascular > Angiogenesis > Adhesion / ECM > Matrix Metalloproteinases > TIMP Cell Biology > Proteolysis / Ubiquitin > Protease inhibitors > Metalloprotease inhibitors > TIMPs Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Enzymes > TIMP1 / TIMP2 |
| 亚细胞定位 | Secreted. |
| 翻译后修饰 | The activity of TIMP1 is dependent on the presence of disulfide bonds. |
| 相似性 | Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain. |
| 功能 | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-500} |
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文献和实验Fluorochrome Absorption and Emission Spectra
. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on stream-in-air flow cytometers. PerCP-Cy5.5 is a tandem conjugate system than combines PerCP with a cyanine dye (Cy5.5™) and has an absorption maximum
Fluorochrome Absorption and Emission
. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on stream-in-air flow cytometers. PerCP-Cy5.5 is a tandem conjugate system than combines PerCP with a cyanine dye (Cy5.5™) and has an absorption maximum
Direct Immunofluorescence Labeling
Recipes Fluorochrome-Conjugated Antibodies: Test antibody: Mouse monoclonal conjugated to FITC, PE, PerCP or PE-CY5 Tandem (tradenames: Cy-Chrome or Tri-Color). If you''re planning to label cells with 2 or more antibodies simultaneously
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