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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
储存条件:-20℃,干燥、避光、密封
- 保质期:
2年
- 英文名:
2-HYDROXY-N-(2-METHOXY-5-NITROPHENYL)BENZYLAMINE
- 库存:
1000
- 供应商:
四川省维克奇生物科技有限公司
- CAS号:
199992-37-5
- 规格:
200mg
英文名称:2-HYDROXY-N-(2-METHOXY-5-NITROPHENYL)BENZYLAMINE
CAS:199992-37-5
储存条件:-20℃,干燥、避光、密封
规格:5mg10mg20mg50mg100mg500mg1g80g等应客户需求包装
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文献和实验Site-directed hydroxy radical mapping of nucleosome positions in vitro
on ice (see note 13); Freshly prepare reagent solution by degassing 3 small sidearm flasks, each containing 50ml water. Add 37.5mg of ferrous ammonium sulphate, 200mg of ascorbic acid and 333µl of hydrogen peroxide to separate flasks. Swirl
as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow the color to develop for 100 minutes and stop reaction by adding 10 µl of 1 N NaOH. Read absorbance
or Costar serocluster "U" vinyl plates #2797). Incubate for 4 hours at 37°C and then store at 4°C until use (if less than two weeks). For the vinyl plates, wash three times with washing solution, dispel liquid by slapping on paper towels and then cover
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