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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
储存条件:-20℃,干燥、避光、密封
- 保质期:
2年
- 英文名:
(1S,3S)-cyclopentane-1,3-diamine;dihydrochloride
- 库存:
1000
- 供应商:
四川省维克奇生物科技有限公司
- CAS号:
2725774-24-1
- 规格:
100mg
英文名称:(1S,3S)-cyclopentane-1,3-diamine;dihydrochloride
分子式:C5H12N2.2HCl
CAS:2725774-24-1
纯度:≥97%
储存条件:-20℃,干燥、避光、密封
规格:5mg10mg20mg50mg100mg500mg1g80g等应客户需求包装
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文献和实验BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
a single colony from a freshly streaked selective plate, and inoculate a starter culture of 2-5 ml LB or YT medium containing the appropriate selective antibiotic. Incubate for ~ 20-24 hr at 37°C with vigorous shaking (~ 300 rpm). Use a flask
E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
实验步骤 1. Isolate a single colony from a freshly streaked selective plate, and inoculate a starter culture of 2-5 ml LB or YT medium containing the appropriate selective antibiotic. Incubate for ~ 20-24 hr at 37°C
BACs, PACs, P1s and Plasmid Isolation Protocol (For low copy number)
-24 hr at 37E C with vigorous shaking (~ 300 rpm). Use a flask with a volume at least 4 times the volume of the culture. 2. Pellet 1.5-5 ml bacteria by centrifugation at 13,000 x g for 3 min at room temperature. Decant or aspirate medium
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