CBZ-7-氨基庚酸 CAS号 23434-37-9

Preparation of Carboxypeptidase Y and Its Properties

℃ for 1 hr. In the presence of 10% methanol, the enzyme is completely stable at pH 5.5～8.0 for 8 hr at 25℃. Aqueous solu­tion of the enzyme is stable for 8 hr in the pH range 5.5～8.0 at 25℃ and for 2 hr in the pH range 6～8 at 37℃. At higher temperatures

A Method for Assaying Deubiquitinating Enzymes

of the purified DUBs, 0.2 mM Cbz-LRGG-AMC, 0.1 M Tris-HCl (pH 7.8), 1 mM EDTA, 1 mM dithiothreitol, and 5% (v/v) glycerol. After incubation of the mixtures for various periods at 37 °C, the reaction was terminated by adding 0.1 ml of 1% (w/v) SDS and 0.8 ml of H2

bsa的性质与作用

相关专题 生物实验中的封闭剂 BSA 的作用 BSA一般做为稳定剂被用于限制酶或者修饰酶的保存溶液和反应液中，因为有些酶在低浓度下不稳定或活性低。加入BSA后，它可能起到“保护”或“载体” 作用，不少酶类添加 BSA后能使其活性大幅度提高。不需要加BSA的酶加入BSA一般不会受到什么影响。对多数底物DNA而言，BSA可以使酶切更完全，并可实现重复切割。在37℃，酶切反应超过1h时，BSA可以使酶更加稳定，因为在不含BSA