Recombinant HTLV-II envelope 8

Recombinant Poxviruses: Versatile Tools for Immunological Assays

several recombinant poxvirus system protocols used to study both MHC class I and class II antigen processing and presentation, as well as provides insight and troubleshooting techniques to improve the reproducibility and fidelity of these experiments.

In Vitro Digestion with Proteases Producing MHC Class II Ligands

Proteases generate peptides that bind to MHC class II molecules to interact with a wide diversity of CD4+ T cells. They are expressed in dedicated organelles: endosomes and lysosomes of professional antigen presenting cells (pAPCs) such as B

DETECTION OF RECOMBINANT PROTEINS BY ELISA

which we use for bacterially expressed recombinant proteins). Controls will include no antigen, and extracts from bacteria transformed with the appropriate expression vector which lack the insert. Add 50µl per well of the appropriate solution in coating buffer