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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
Polyclonal
- 抗体名:
Cy3标记的剪接调控蛋白129抗体
- 宿主:
Rabbit
- 形态:
Lyophilized or Liquid
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20°C
- 规格:
100ul
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文献和实验Staining for Surface Receptors
up" on the coverslip, and the wet whatman paper helps prevent the antibody from drying up. 9. Aspirate Blotto, and apply secondary Ab. Incubate 20' in the dark. Dilute antibody with Blotto. Cy3 donkey anti-mouse (Jackson Immunoresearch ) 1:500, FITC goat anti-mouse
)1:1000. 9. Wash 3x. 10. Second Blotto block as above, 5'. 11. Aspirate Blotto, and apply secondary Ab. Incubate 20' in the dark. Dilute antibody with Blotto. Cy3 donkey anti-mouse (Jackson Immunoresearch ) 1:500, FITC goat anti-mouse 1:500
1767, and Cy3-BSA-AP1780. Anti-DIG, streptavidin, and FKBP12 were spotted in quadruplicate on aldehyde slides and processed as described above. To probe the slides, the doubly labeled BSA conjugates were diluted into PBST supplemented with 1% BSA
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