- ￥220
- BIOSS
- ZK-0122378
- 中国/美国
- 2025年11月21日
- ICC=1:50-200 IF=1:50-200
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
Polyclonal
- 抗体名：
辣根过氧化物酶标记的同源盒蛋白SIX4抗体
- 宿主：
Rabbit
- 形态：
Lyophilized or Liquid
- 应用范围：
ICC=1:50-200 IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20°C
- 规格：
100ul
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
anti-mouse HRP antibody diluted 1:80 for 40 minutes at 37C We use DAKO P0260 (12) Wash with blocking solution for 10 minutes (13) Incubate with swine anti-rabbit HRP antibody diluted 1:100 for 40 minutes at 37C We use DAKO P0217 (14
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
Wholemount staining of embryos
as above 15 to 30''. Secondary antibody is added in blocking solution. Concentration varies, but 1/500 works for most. For monoclonal primaries use anti-mouse coupled to AP or HRP, for polyclonals use anti-rabbit coupled AP or HRP. Incubate at 4 degC
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