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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
再康生物
- 样本:
血清液/组织/尿
- 库存:
大量
- 标记物:
见 说 明 书
- 适应物种:
Human/Mouse/Rat
- 应用:
科学研究
- 检测方法:
Western blot/Elisa/IHC
- 检测范围:
见 说 明 书
- 规格:
4 pack
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
and to use PCR to determine that the cells contain your construct. PCR is necessary here because the Ti plasmid is single copy and you can barely see it on agarose gels. 1. Grow cells overnight in 5 ml LB or YEP with antibiotics. For pMONs in ABI - 50
. In the first, ROCK inhibitor (ROCKi) was used, which allows accurate counting and plating of single hESC. In the second, small colonies were plated without ROCKi but with adaptations for accurate counting and plating. The MTT assay was also adapted for use with mouse
Co-Expression of human DNA primase p49-p58 subunits
Adapted from W.C.Copeland,Protein Exp.& Purif 9,(1997)1-9 and Schneider,et.al.,JBC 273:34 (1998)21608-21615. Obtained from Fanning lab and revised by LSM,7/23/01 Obtained cell stock of BL21(DE3)cells cotransformed with pET21a-Hp49 (ampicillin
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