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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
Polyclonal
- 抗体名:
FITC标记的酪蛋白激酶1/casein kinase Iα抗体
- 宿主:
Rabbit
- 形态:
Lyophilized or Liquid
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20°C
- 规格:
100ul
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文献和实验Immunofluorescent Localization of Tubulin
Rabbit anti-tubulin (or other primary antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter
antibodies in 1% NGS/CMF-PBS. Make 4 mls anti-mouse FTIC diluted 1:150 Make 1.5 mls anti-rabbit FITC diluted 1:200 6. Bind secondary antibodies 45 minutes--use 300 ul/well. 7. Wash 3X with PBST, 5 minutes each wash-use ~1 ml/well. 8.
Cell Surface Immunofluorescence Staining Protocol
as the antibodies used for immunofluorescent staining. 4. Cell-Surface Staining with Antibody: Add appropriately conjugated fluorescent, biotinylated, or purified primary antibodies at predetermined optimum concentrations (e.g. anti-CD3-FITC, anti
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