pHSREM1

质粒编号:
质粒名称: pHSREM1
其他名称:
质粒大小（bp）: 3400
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: A KpnI/SacI double digest releases the hsp70 transcription module, for subsequent ligation into other plasmids such as ones containing Drosophila P elements. KpnI BglII NotI XhoI – promoter – MCS – terminator – SpeI XbaI NotI EagI BstXI SacII SacI. One of a series (ATCC 37642-37645) of plasmid vectors for expressing genes under the control of the Drosophila hsp70 promoter & terminator. The transcription module is flanked by restriction sites (facilitating its removal), T7 and T3 promoters (for in vitro RNA synthesis), & primers for Sanger sequencing. There is also an M13 origin of replication. Restriction digests of the clone give the following sizes (kb): PstI–3.4; HindIII–3.3; EcoRI–3.4; BamHI–3.3. (ATCC staff) BamHI/BglII fragment of pCIII1 with terminator plus several upstream sites of Bluescript MCS ligated into BamHI of pCII38 = pCIII8. KpnI/XhoI region of pCIII8 replaced with synthetic adapter with NotI and BglII sites = pHSREM1. (personal communication) XhoI/HindIII fragment of Drosophila hsp70 with nt -89 to +89 (relative to transcription start site) ligated to XhoI/HindIII Bluescript KS+ = pCII38. 248 bp SalI/XhoI fragment with 3' end of hsp70 ligated into SalI of Bluescript KS+ = pCIII1. (personal communication) Major Drosophila hsp70 regulator elements are: 2 heat shock consensus elements (-85 to -72 & -62 to -49),a TATA box (-33 to -26) & transcription initiation site (+1) in the 5' segment; the termination triplet and polyA site in the 3' segment. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli.
质粒图谱:

质粒序列: