- 详细信息
- 技术资料
- 库存:
大量
- 英文名:
pET-41Ek/LIC
- 保质期:
1年
- 供应商:
再康生物
- 保存条件:
低温运输,-20℃保存
质粒编号: 60908-2806
质粒名称: pET-41Ek/LIC
其他名称:
质粒大小(bp): 5947
抗性: Kanamycin
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: GST (Nterm), His (Nterm), S-Tag (Cterm)
5’测序引物:T7 Fwd
5′ 测序引物序列:5’d[TAATACGACTCACTATAGGG]3′
GenBank号:
其他属性: For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 120 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| EK | 293 | 279 | |
| S15 | 368 | 324 | |
| 6xHIS | 428 | 411 | |
| GST (variant) | 1109 | 459 | |
| T7_transl_en_RBS | 1134 | 1118 | |
| lacO | 1179 | 1152 | |
| T7_promoter | 1197 | 1179 | |
| tet (300 – 563) | 1233 | 1496 | |
| pBRrevBam_primer | 1304 | 1285 | |
| lacI | 1579 | 2670 | |
| ROP | 3242 | 3433 | |
| pGEX_3_primer | 3449 | 3427 | |
| pBR322_origin | 4467 | 3848 | |
| KanR2 | 4573 | 5388 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 1109 | 147 | |
| ORF frame 3 | 1110 | 1838 | |
| ORF frame 1 | 1711 | 2670 | |
| ORF frame 1 | 4573 | 5388 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 174 |
| NotI | 182 |
| EagI | 182 |
| HindIII | 189 |
| SalI | 195 |
| SacI | 206 |
| PstI | 214 |
| AscI | 216 |
| StuI | 226 |
| EcoRI | 235 |
| BamHI | 241 |
| EcoRV | 251 |
| NcoI | 254 |
| AgeI | 306 |
| KpnI | 313 |
| BglII | 316 |
| SacII | 407 |
| SpeI | 438 |
| MscI | 901 |
| NdeI | 1108 |
| XbaI | 1146 |
| ApaI | 2149 |
| HpaI | 2444 |
| NruI | 4661 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgtttagcag | cctaggtatt | aatcaattag | 150 |
| 151 | tggtggtggt | ggtggtggtg | gtgctcgagt | gcggccgcaa | gcttgtcgac | 200 |
| 201 | ggagctcgcc | tgcaggcgcg | ccaaggcctg | tacagaattc | ggatccccga | 250 |
| 251 | tatccatggt | tgaggagaag | cccgggctct | tgtcgtcgtc | atcaccagaa | 300 |
| 301 | ccaccaccgg | tacccagatc | tgggctgtcc | atgtgctggc | gttcgaattt | 350 |
| 351 | agcagcagcg | gtttctttca | taccaattgc | agtactaccg | cgtggcacca | 400 |
| 401 | gacccgcgga | gtgatggtga | tggtgatgac | cagaaccact | agttgaacca | 450 |
| 451 | tccgattttg | gaggatggtc | gccaccacca | aacgtggctt | gccagccctg | 500 |
| 501 | caaaggccat | gctatatact | tgctggattt | caagtactta | tcaatttgtg | 550 |
| 551 | ggatagcttc | aatacgtttt | ttaaaacaaa | ctaattttgg | gaacgcatcc | 600 |
| 601 | aggcacattg | ggtccatgta | taaaacaaca | tcaagagcgt | catacaacat | 650 |
| 651 | gaagtcagga | tgggttacat | gatcaccatt | taaatatgtt | ttatgacata | 700 |
| 701 | aacgatcttc | gaacattttc | agcatttcag | gtagcttgct | aagaaaatca | 750 |
| 751 | actttgagag | tttcaaagtc | tttactatat | gcaattctcg | aaacaccgta | 800 |
| 801 | tctaatatcc | aaaaccgctc | cttcaagcat | tgaaatctct | gcacgctctt | 850 |
| 851 | ttggacaacc | acccaacatg | ttgtgcttgt | cagctatata | acgtatgatg | 900 |
| 901 | gccatagact | gtgttaattt | aacatcacca | tcaatataat | aaggaagatt | 950 |
| 951 | gggaaactcc | aaacccaatt | caaacttttt | gtttcgccat | ttatcacctt | 1000 |
| 1001 | catcgcgctc | atacaaatgc | tcttcatatt | tttcttcaag | atattccaaa | 1050 |
| 1051 | agaagtcgag | tgggttgcac | aaggccctta | attttccaat | aacctagtat | 1100 |
| 1101 | aggggacata | tgtatatctc | cttcttaaag | ttaaacaaaa | ttatttctag | 1150 |
| 1151 | aggggaattg | ttatccgctc | acaattcccc | tatagtgagt | cgtattaatt | 1200 |
| 1201 | tcgcgggatc | gagatcgatc | tcgatcctct | acgccggacg | catcgtggcc | 1250 |
| 1251 | ggcatcaccg | gcgccacagg | tgcggttgct | ggcgcctata | tcgccgacat | 1300 |
| 1301 | caccgatggg | gaagatcggg | ctcgccactt | cgggctcatg | agcgcttgtt | 1350 |
| 1351 | tcggcgtggg | tatggtggca | ggccccgtgg | ccgggggact | gttgggcgcc | 1400 |
| 1401 | atctccttgc | atgcaccatt | ccttgcggcg | gcggtgctca | acggcctcaa | 1450 |
| 1451 | cctactactg | ggctgcttcc | taatgcagga | gtcgcataag | ggagagcgtc | 1500 |
| 1501 | gagatcccgg | acaccatcga | atggcgcaaa | acctttcgcg | gtatggcatg | 1550 |
| 1551 | atagcgcccg | gaagagagtc | aattcagggt | ggtgaatgtg | aaaccagtaa | 1600 |
| 1601 | cgttatacga | tgtcgcagag | tatgccggtg | tctcttatca | gaccgtttcc | 1650 |
| 1651 | cgcgtggtga | accaggccag | ccacgtttct | gcgaaaacgc | gggaaaaagt | 1700 |
| 1701 | ggaagcggcg | atggcggagc | tgaattacat | tcccaaccgc | gtggcacaac | 1750 |
| 1751 | aactggcggg | caaacagtcg | ttgctgattg | gcgttgccac | ctccagtctg | 1800 |
| 1801 | gccctgcacg | cgccgtcgca | aattgtcgcg | gcgattaaat | ctcgcgccga | 1850 |
| 1851 | tcaactgggt | gccagcgtgg | tggtgtcgat | ggtagaacga | agcggcgtcg | 1900 |
| 1901 | aagcctgtaa | agcggcggtg | cacaatcttc | tcgcgcaacg | cgtcagtggg | 1950 |
| 1951 | ctgatcatta | actatccgct | ggatgaccag | gatgccattg | ctgtggaagc | 2000 |
| 2001 | tgcctgcact | aatgttccgg | cgttatttct | tgatgtctct | gaccagacac | 2050 |
| 2051 | ccatcaacag | tattattttc | tcccatgaag | acggtacgcg | actgggcgtg | 2100 |
| 2101 | gagcatctgg | tcgcattggg | tcaccagcaa | atcgcgctgt | tagcgggccc | 2150 |
| 2151 | attaagttct | gtctcggcgc | gtctgcgtct | ggctggctgg | cataaatatc | 2200 |
| 2201 | tcactcgcaa | tcaaattcag | ccgatagcgg | aacgggaagg | cgactggagt | 2250 |
| 2251 | gccatgtccg | gttttcaaca | aaccatgcaa | atgctgaatg | agggcatcgt | 2300 |
| 2301 | tcccactgcg | atgctggttg | ccaacgatca | gatggcgctg | ggcgcaatgc | 2350 |
| 2351 | gcgccattac | cgagtccggg | ctgcgcgttg | gtgcggacat | ctcggtagtg | 2400 |
| 2401 | ggatacgacg | ataccgaaga | cagctcatgt | tatatcccgc | cgttaaccac | 2450 |
| 2451 | catcaaacag | gattttcgcc | tgctggggca | aaccagcgtg | gaccgcttgc | 2500 |
| 2501 | tgcaactctc | tcagggccag | gcggtgaagg | gcaatcagct | gttgcccgtc | 2550 |
| 2551 | tcactggtga | aaagaaaaac | caccctggcg | cccaatacgc | aaaccgcctc | 2600 |
| 2601 | tccccgcgcg | ttggccgatt | cattaatgca | gctggcacga | caggtttccc | 2650 |
| 2651 | gactggaaag | cgggcagtga | gcgcaacgca | attaatgtaa | gttagctcac | 2700 |
| 2701 | tcattaggca | ccgggatctc | gaccgatgcc | cttgagagcc | ttcaacccag | 2750 |
| 2751 | tcagctcctt | ccggtgggcg | cggggcatga | ctagcatgat | cgtgctcctg | 2800 |
| 2801 | tcgttgagga | cccggctagg | ctggcggggt | tgccttactg | gttagcagaa | 2850 |
| 2851 | tgaatcaccg | atacgcgagc | gaacgtgaag | cgactgctgc | tgcaaaacgt | 2900 |
| 2901 | ctgcgacctg | agcaacaaca | tgaatggtct | tcggtttccg | tgtttcgtaa | 2950 |
| 2951 | agtctggaaa | cgcggaagtc | agcgccctgc | accattatgt | tccggatctg | 3000 |
| 3001 | catcgcagga | tgctgctggc | taccctgtgg | aacacctaca | tctgtattaa | 3050 |
| 3051 | cgaagcgctg | gcattgaccc | tgagtgattt | ttctctggtc | ccgccgcatc | 3100 |
| 3101 | cataccgcca | gttgtttacc | ctcacaacgt | tccagtaacc | gggcatgttc | 3150 |
| 3151 | atcatcagta | acccgtatcg | tgagcatcct | ctctcgtttc | atcggtatca | 3200 |
| 3201 | ttacccccat | gaacagaaat | cccccttaca | cggaggcatc | agtgaccaaa | 3250 |
| 3251 | caggaaaaaa | ccgcccttaa | catggcccgc | tttatcagaa | gccagacatt | 3300 |
| 3301 | aacgcttctg | gagaaactca | acgagctgga | cgcggatgaa | caggcagaca | 3350 |
| 3351 | tctgtgaatc | gcttcacgac | cacgctgatg | agctttaccg | cagctgcctc | 3400 |
| 3401 | gcgcgtttcg | gtgatgacgg | tgaaaacctc | tgacacatgc | agctcccgga | 3450 |
| 3451 | gacggtcaca | gcttgtctgt | aagcggatgc | cgggagcaga | caagcccgtc | 3500 |
| 3501 | agggcgcgtc | agcgggtgtt | ggcgggtgtc | ggggcgcagc | catgacccag | 3550 |
| 3551 | tcacgtagcg | atagcggagt | gtatactggc | ttaactatgc | ggcatcagag | 3600 |
| 3601 | cagattgtac | tgagagtgca | ccatatatgc | ggtgtgaaat | accgcacaga | 3650 |
| 3651 | tgcgtaagga | gaaaataccg | catcaggcgc | tcttccgctt | cctcgctcac | 3700 |
| 3701 | tgactcgctg | cgctcggtcg | ttcggctgcg | gcgagcggta | tcagctcact | 3750 |
| 3751 | caaaggcggt | aatacggtta | tccacagaat | caggggataa | cgcaggaaag | 3800 |
| 3801 | aacatgtgag | caaaaggcca | gcaaaaggcc | aggaaccgta | aaaaggccgc | 3850 |
| 3851 | gttgctggcg | tttttccata | ggctccgccc | ccctgacgag | catcacaaaa | 3900 |
| 3901 | atcgacgctc | aagtcagagg | tggcgaaacc | cgacaggact | ataaagatac | 3950 |
| 3951 | caggcgtttc | cccctggaag | ctccctcgtg | cgctctcctg | ttccgaccct | 4000 |
| 4001 | gccgcttacc | ggatacctgt | ccgcctttct | cccttcggga | agcgtggcgc | 4050 |
| 4051 | tttctcatag | ctcacgctgt | aggtatctca | gttcggtgta | ggtcgttcgc | 4100 |
| 4101 | tccaagctgg | gctgtgtgca | cgaacccccc | gttcagcccg | accgctgcgc | 4150 |
| 4151 | cttatccggt | aactatcgtc | ttgagtccaa | cccggtaaga | cacgacttat | 4200 |
| 4201 | cgccactggc | agcagccact | ggtaacagga | ttagcagagc | gaggtatgta | 4250 |
| 4251 | ggcggtgcta | cagagttctt | gaagtggtgg | cctaactacg | gctacactag | 4300 |
| 4301 | aaggacagta | tttggtatct | gcgctctgct | gaagccagtt | accttcggaa | 4350 |
| 4351 | aaagagttgg | tagctcttga | tccggcaaac | aaaccaccgc | tggtagcggt | 4400 |
| 4401 | ggtttttttg | tttgcaagca | gcagattacg | cgcagaaaaa | aaggatctca | 4450 |
| 4451 | agaagatcct | ttgatctttt | ctacggggtc | tgacgctcag | tggaacgaaa | 4500 |
| 4501 | actcacgtta | agggattttg | gtcatgaaca | ataaaactgt | ctgcttacat | 4550 |
| 4551 | aaacagtaat | acaaggggtg | ttatgagcca | tattcaacgg | gaaacgtctt | 4600 |
| 4601 | gctctaggcc | gcgattaaat | tccaacatgg | atgctgattt | atatgggtat | 4650 |
| 4651 | aaatgggctc | gcgataatgt | cgggcaatca | ggtgcgacaa | tctatcgatt | 4700 |
| 4701 | gtatgggaag | cccgatgcgc | cagagttgtt | tctgaaacat | ggcaaaggta | 4750 |
| 4751 | gcgttgccaa | tgatgttaca | gatgagatgg | tcagactaaa | ctggctgacg | 4800 |
| 4801 | gaatttatgc | ctcttccgac | catcaagcat | tttatccgta | ctcctgatga | 4850 |
| 4851 | tgcatggtta | ctcaccactg | cgatccccgg | gaaaacagca | ttccaggtat | 4900 |
| 4901 | tagaagaata | tcctgattca | ggtgaaaata | ttgttgatgc | gctggcagtg | 4950 |
| 4951 | ttcctgcgcc | ggttgcattc | gattcctgtt | tgtaattgtc | cttttaacag | 5000 |
| 5001 | cgatcgcgta | tttcgtctcg | ctcaggcgca | atcacgaatg | aataacggtt | 5050 |
| 5051 | tggttgatgc | gagtgatttt | gatgacgagc | gtaatggctg | gcctgttgaa | 5100 |
| 5101 | caagtctgga | aagaaatgca | taaacttttg | ccattctcac | cggattcagt | 5150 |
| 5151 | cgtcactcat | ggtgatttct | cacttgataa | ccttattttt | gacgagggga | 5200 |
| 5201 | aattaatagg | ttgtattgat | gttggacgag | tcggaatcgc | agaccgatac | 5250 |
| 5251 | caggatcttg | ccatcctatg | gaactgcctc | ggtgagtttt | ctccttcatt | 5300 |
| 5301 | acagaaacgg | ctttttcaaa | aatatggtat | tgataatcct | gatatgaata | 5350 |
| 5351 | aattgcagtt | tcatttgatg | ctcgatgagt | ttttctaaga | attaattcat | 5400 |
| 5401 | gagcggatac | atatttgaat | gtatttagaa | aaataaacaa | ataggggttc | 5450 |
| 5451 | cgcgcacatt | tccccgaaaa | gtgccacctg | aaattgtaaa | cgttaatatt | 5500 |
| 5501 | ttgttaaaat | tcgcgttaaa | tttttgttaa | atcagctcat | tttttaacca | 5550 |
| 5551 | ataggccgaa | atcggcaaaa | tcccttataa | atcaaaagaa | tagaccgaga | 5600 |
| 5601 | tagggttgag | tgttgttcca | gtttggaaca | agagtccact | attaaagaac | 5650 |
| 5651 | gtggactcca | acgtcaaagg | gcgaaaaacc | gtctatcagg | gcgatggccc | 5700 |
| 5701 | actacgtgaa | ccatcaccct | aatcaagttt | tttggggtcg | aggtgccgta | 5750 |
| 5751 | aagcactaaa | tcggaaccct | aaagggagcc | cccgatttag | agcttgacgg | 5800 |
| 5801 | ggaaagccgg | cgaacgtggc | gagaaaggaa | gggaagaaag | cgaaaggagc | 5850 |
| 5851 | gggcgctagg | gcgctggcaa | gtgtagcggt | cacgctgcgc | gtaaccacca | 5900 |
| 5901 | cacccgccgc | gcttaatgcg | ccgctacagg | gcgcgtccca | ttcgcca | 5947 |
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pET-41Ek/LIC
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