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- ZK-0023677
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- 2025年11月21日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-42a(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2808y
质粒名称: pET-42a(+)
其他名称:
质粒大小（bp）: 5930
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-GST, N-His, N-S, C-His, N-Thrombin, N-Factor Xa
5′ 测序引物:T7或pGEX-5‘
5′ 测序引物序列: T7: 5′-TAATACGACTCACTATAGGG-3′; pGEX-5′: 5′-GGGCTGGCAAGCCACGTTTGGTG-3′
GenBank号:
其他属性:
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 120 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| S15 | 351 | 307 | |
| 6xHIS | 411 | 394 | |
| GST (variant) | 1092 | 442 | |
| T7_transl_en_RBS | 1117 | 1101 | |
| lacO | 1162 | 1135 | |
| T7_promoter | 1180 | 1162 | |
| tet (300 – 563) | 1216 | 1479 | |
| pBRrevBam_primer | 1287 | 1268 | |
| lacI | 1562 | 2653 | |
| ROP | 3225 | 3416 | |
| pGEX_3_primer | 3432 | 3410 | |
| pBR322_origin | 4450 | 3831 | |
| KanR2 | 4556 | 5371 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 1092 | 139 | |
| ORF frame 1 | 1093 | 1821 | |
| ORF frame 2 | 1694 | 2653 | |
| ORF frame 2 | 4556 | 5371 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 174 |
| NotI | 182 |
| EagI | 182 |
| HindIII | 189 |
| SalI | 195 |
| SacI | 206 |
| PstI | 214 |
| AscI | 216 |
| StuI | 226 |
| EcoRI | 235 |
| BamHI | 241 |
| EcoRV | 251 |
| NcoI | 255 |
| AgeI | 289 |
| KpnI | 296 |
| BglII | 299 |
| SacII | 390 |
| SpeI | 421 |
| MscI | 884 |
| NdeI | 1091 |
| XbaI | 1129 |
| ApaI | 2132 |
| HpaI | 2427 |
| NruI | 4644 |
| SmaI | 4861 |
| XmaI | 4859 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgtttagcag | cctaggtatt | aatcaattag | 150 |
| 151 | tggtggtggt | ggtggtggtg | gtgctcgagt | gcggccgcaa | gcttgtcgac | 200 |
| 201 | ggagctcgcc | tgcaggcgcg | ccaaggcctg | tacagaattc | ggatccccga | 250 |
| 251 | tatcccatgg | acccgcgtcc | ctcaataccg | gagccaccac | cggtacccag | 300 |
| 301 | atctgggctg | tccatgtgct | ggcgttcgaa | tttagcagca | gcggtttctt | 350 |
| 351 | tcataccaat | tgcagtacta | ccgcgtggca | ccagacccgc | ggagtgatgg | 400 |
| 401 | tgatggtgat | gaccagaacc | actagttgaa | ccatccgatt | ttggaggatg | 450 |
| 451 | gtcgccacca | ccaaacgtgg | cttgccagcc | ctgcaaaggc | catgctatat | 500 |
| 501 | acttgctgga | tttcaagtac | ttatcaattt | gtgggatagc | ttcaatacgt | 550 |
| 551 | tttttaaaac | aaactaattt | tgggaacgca | tccaggcaca | ttgggtccat | 600 |
| 601 | gtataaaaca | acatcaagag | cgtcatacaa | catgaagtca | ggatgggtta | 650 |
| 651 | catgatcacc | atttaaatat | gttttatgac | ataaacgatc | ttcgaacatt | 700 |
| 701 | ttcagcattt | caggtagctt | gctaagaaaa | tcaactttga | gagtttcaaa | 750 |
| 751 | gtctttacta | tatgcaattc | tcgaaacacc | gtatctaata | tccaaaaccg | 800 |
| 801 | ctccttcaag | cattgaaatc | tctgcacgct | cttttggaca | accacccaac | 850 |
| 851 | atgttgtgct | tgtcagctat | ataacgtatg | atggccatag | actgtgttaa | 900 |
| 901 | tttaacatca | ccatcaatat | aataaggaag | attgggaaac | tccaaaccca | 950 |
| 951 | attcaaactt | tttgtttcgc | catttatcac | cttcatcgcg | ctcatacaaa | 1000 |
| 1001 | tgctcttcat | atttttcttc | aagatattcc | aaaagaagtc | gagtgggttg | 1050 |
| 1051 | cacaaggccc | ttaattttcc | aataacctag | tataggggac | atatgtatat | 1100 |
| 1101 | ctccttctta | aagttaaaca | aaattatttc | tagaggggaa | ttgttatccg | 1150 |
| 1151 | ctcacaattc | ccctatagtg | agtcgtatta | atttcgcggg | atcgagatcg | 1200 |
| 1201 | atctcgatcc | tctacgccgg | acgcatcgtg | gccggcatca | ccggcgccac | 1250 |
| 1251 | aggtgcggtt | gctggcgcct | atatcgccga | catcaccgat | ggggaagatc | 1300 |
| 1301 | gggctcgcca | cttcgggctc | atgagcgctt | gtttcggcgt | gggtatggtg | 1350 |
| 1351 | gcaggccccg | tggccggggg | actgttgggc | gccatctcct | tgcatgcacc | 1400 |
| 1401 | attccttgcg | gcggcggtgc | tcaacggcct | caacctacta | ctgggctgct | 1450 |
| 1451 | tcctaatgca | ggagtcgcat | aagggagagc | gtcgagatcc | cggacaccat | 1500 |
| 1501 | cgaatggcgc | aaaacctttc | gcggtatggc | atgatagcgc | ccggaagaga | 1550 |
| 1551 | gtcaattcag | ggtggtgaat | gtgaaaccag | taacgttata | cgatgtcgca | 1600 |
| 1601 | gagtatgccg | gtgtctctta | tcagaccgtt | tcccgcgtgg | tgaaccaggc | 1650 |
| 1651 | cagccacgtt | tctgcgaaaa | cgcgggaaaa | agtggaagcg | gcgatggcgg | 1700 |
| 1701 | agctgaatta | cattcccaac | cgcgtggcac | aacaactggc | gggcaaacag | 1750 |
| 1751 | tcgttgctga | ttggcgttgc | cacctccagt | ctggccctgc | acgcgccgtc | 1800 |
| 1801 | gcaaattgtc | gcggcgatta | aatctcgcgc | cgatcaactg | ggtgccagcg | 1850 |
| 1851 | tggtggtgtc | gatggtagaa | cgaagcggcg | tcgaagcctg | taaagcggcg | 1900 |
| 1901 | gtgcacaatc | ttctcgcgca | acgcgtcagt | gggctgatca | ttaactatcc | 1950 |
| 1951 | gctggatgac | caggatgcca | ttgctgtgga | agctgcctgc | actaatgttc | 2000 |
| 2001 | cggcgttatt | tcttgatgtc | tctgaccaga | cacccatcaa | cagtattatt | 2050 |
| 2051 | ttctcccatg | aagacggtac | gcgactgggc | gtggagcatc | tggtcgcatt | 2100 |
| 2101 | gggtcaccag | caaatcgcgc | tgttagcggg | cccattaagt | tctgtctcgg | 2150 |
| 2151 | cgcgtctgcg | tctggctggc | tggcataaat | atctcactcg | caatcaaatt | 2200 |
| 2201 | cagccgatag | cggaacggga | aggcgactgg | agtgccatgt | ccggttttca | 2250 |
| 2251 | acaaaccatg | caaatgctga | atgagggcat | cgttcccact | gcgatgctgg | 2300 |
| 2301 | ttgccaacga | tcagatggcg | ctgggcgcaa | tgcgcgccat | taccgagtcc | 2350 |
| 2351 | gggctgcgcg | ttggtgcgga | catctcggta | gtgggatacg | acgataccga | 2400 |
| 2401 | agacagctca | tgttatatcc | cgccgttaac | caccatcaaa | caggattttc | 2450 |
| 2451 | gcctgctggg | gcaaaccagc | gtggaccgct | tgctgcaact | ctctcagggc | 2500 |
| 2501 | caggcggtga | agggcaatca | gctgttgccc | gtctcactgg | tgaaaagaaa | 2550 |
| 2551 | aaccaccctg | gcgcccaata | cgcaaaccgc | ctctccccgc | gcgttggccg | 2600 |
| 2601 | attcattaat | gcagctggca | cgacaggttt | cccgactgga | aagcgggcag | 2650 |
| 2651 | tgagcgcaac | gcaattaatg | taagttagct | cactcattag | gcaccgggat | 2700 |
| 2701 | ctcgaccgat | gcccttgaga | gccttcaacc | cagtcagctc | cttccggtgg | 2750 |
| 2751 | gcgcggggca | tgactagcat | gatcgtgctc | ctgtcgttga | ggacccggct | 2800 |
| 2801 | aggctggcgg | ggttgcctta | ctggttagca | gaatgaatca | ccgatacgcg | 2850 |
| 2851 | agcgaacgtg | aagcgactgc | tgctgcaaaa | cgtctgcgac | ctgagcaaca | 2900 |
| 2901 | acatgaatgg | tcttcggttt | ccgtgtttcg | taaagtctgg | aaacgcggaa | 2950 |
| 2951 | gtcagcgccc | tgcaccatta | tgttccggat | ctgcatcgca | ggatgctgct | 3000 |
| 3001 | ggctaccctg | tggaacacct | acatctgtat | taacgaagcg | ctggcattga | 3050 |
| 3051 | ccctgagtga | tttttctctg | gtcccgccgc | atccataccg | ccagttgttt | 3100 |
| 3101 | accctcacaa | cgttccagta | accgggcatg | ttcatcatca | gtaacccgta | 3150 |
| 3151 | tcgtgagcat | cctctctcgt | ttcatcggta | tcattacccc | catgaacaga | 3200 |
| 3201 | aatccccctt | acacggaggc | atcagtgacc | aaacaggaaa | aaaccgccct | 3250 |
| 3251 | taacatggcc | cgctttatca | gaagccagac | attaacgctt | ctggagaaac | 3300 |
| 3301 | tcaacgagct | ggacgcggat | gaacaggcag | acatctgtga | atcgcttcac | 3350 |
| 3351 | gaccacgctg | atgagcttta | ccgcagctgc | ctcgcgcgtt | tcggtgatga | 3400 |
| 3401 | cggtgaaaac | ctctgacaca | tgcagctccc | ggagacggtc | acagcttgtc | 3450 |
| 3451 | tgtaagcgga | tgccgggagc | agacaagccc | gtcagggcgc | gtcagcgggt | 3500 |
| 3501 | gttggcgggt | gtcggggcgc | agccatgacc | cagtcacgta | gcgatagcgg | 3550 |
| 3551 | agtgtatact | ggcttaacta | tgcggcatca | gagcagattg | tactgagagt | 3600 |
| 3601 | gcaccatata | tgcggtgtga | aataccgcac | agatgcgtaa | ggagaaaata | 3650 |
| 3651 | ccgcatcagg | cgctcttccg | cttcctcgct | cactgactcg | ctgcgctcgg | 3700 |
| 3701 | tcgttcggct | gcggcgagcg | gtatcagctc | actcaaaggc | ggtaatacgg | 3750 |
| 3751 | ttatccacag | aatcagggga | taacgcagga | aagaacatgt | gagcaaaagg | 3800 |
| 3801 | ccagcaaaag | gccaggaacc | gtaaaaaggc | cgcgttgctg | gcgtttttcc | 3850 |
| 3851 | ataggctccg | cccccctgac | gagcatcaca | aaaatcgacg | ctcaagtcag | 3900 |
| 3901 | aggtggcgaa | acccgacagg | actataaaga | taccaggcgt | ttccccctgg | 3950 |
| 3951 | aagctccctc | gtgcgctctc | ctgttccgac | cctgccgctt | accggatacc | 4000 |
| 4001 | tgtccgcctt | tctcccttcg | ggaagcgtgg | cgctttctca | tagctcacgc | 4050 |
| 4051 | tgtaggtatc | tcagttcggt | gtaggtcgtt | cgctccaagc | tgggctgtgt | 4100 |
| 4101 | gcacgaaccc | cccgttcagc | ccgaccgctg | cgccttatcc | ggtaactatc | 4150 |
| 4151 | gtcttgagtc | caacccggta | agacacgact | tatcgccact | ggcagcagcc | 4200 |
| 4201 | actggtaaca | ggattagcag | agcgaggtat | gtaggcggtg | ctacagagtt | 4250 |
| 4251 | cttgaagtgg | tggcctaact | acggctacac | tagaaggaca | gtatttggta | 4300 |
| 4301 | tctgcgctct | gctgaagcca | gttaccttcg | gaaaaagagt | tggtagctct | 4350 |
| 4351 | tgatccggca | aacaaaccac | cgctggtagc | ggtggttttt | ttgtttgcaa | 4400 |
| 4401 | gcagcagatt | acgcgcagaa | aaaaaggatc | tcaagaagat | cctttgatct | 4450 |
| 4451 | tttctacggg | gtctgacgct | cagtggaacg | aaaactcacg | ttaagggatt | 4500 |
| 4501 | ttggtcatga | acaataaaac | tgtctgctta | cataaacagt | aatacaaggg | 4550 |
| 4551 | gtgttatgag | ccatattcaa | cgggaaacgt | cttgctctag | gccgcgatta | 4600 |
| 4601 | aattccaaca | tggatgctga | tttatatggg | tataaatggg | ctcgcgataa | 4650 |
| 4651 | tgtcgggcaa | tcaggtgcga | caatctatcg | attgtatggg | aagcccgatg | 4700 |
| 4701 | cgccagagtt | gtttctgaaa | catggcaaag | gtagcgttgc | caatgatgtt | 4750 |
| 4751 | acagatgaga | tggtcagact | aaactggctg | acggaattta | tgcctcttcc | 4800 |
| 4801 | gaccatcaag | cattttatcc | gtactcctga | tgatgcatgg | ttactcacca | 4850 |
| 4851 | ctgcgatccc | cgggaaaaca | gcattccagg | tattagaaga | atatcctgat | 4900 |
| 4901 | tcaggtgaaa | atattgttga | tgcgctggca | gtgttcctgc | gccggttgca | 4950 |
| 4951 | ttcgattcct | gtttgtaatt | gtccttttaa | cagcgatcgc | gtatttcgtc | 5000 |
| 5001 | tcgctcaggc | gcaatcacga | atgaataacg | gtttggttga | tgcgagtgat | 5050 |
| 5051 | tttgatgacg | agcgtaatgg | ctggcctgtt | gaacaagtct | ggaaagaaat | 5100 |
| 5101 | gcataaactt | ttgccattct | caccggattc | agtcgtcact | catggtgatt | 5150 |
| 5151 | tctcacttga | taaccttatt | tttgacgagg | ggaaattaat | aggttgtatt | 5200 |
| 5201 | gatgttggac | gagtcggaat | cgcagaccga | taccaggatc | ttgccatcct | 5250 |
| 5251 | atggaactgc | ctcggtgagt | tttctccttc | attacagaaa | cggctttttc | 5300 |
| 5301 | aaaaatatgg | tattgataat | cctgatatga | ataaattgca | gtttcatttg | 5350 |
| 5351 | atgctcgatg | agtttttcta | agaattaatt | catgagcgga | tacatatttg | 5400 |
| 5401 | aatgtattta | gaaaaataaa | caaatagggg | ttccgcgcac | atttccccga | 5450 |
| 5451 | aaagtgccac | ctgaaattgt | aaacgttaat | attttgttaa | aattcgcgtt | 5500 |
| 5501 | aaatttttgt | taaatcagct | cattttttaa | ccaataggcc | gaaatcggca | 5550 |
| 5551 | aaatccctta | taaatcaaaa | gaatagaccg | agatagggtt | gagtgttgtt | 5600 |
| 5601 | ccagtttgga | acaagagtcc | actattaaag | aacgtggact | ccaacgtcaa | 5650 |
| 5651 | agggcgaaaa | accgtctatc | agggcgatgg | cccactacgt | gaaccatcac | 5700 |
| 5701 | cctaatcaag | ttttttgggg | tcgaggtgcc | gtaaagcact | aaatcggaac | 5750 |
| 5751 | cctaaaggga | gcccccgatt | tagagcttga | cggggaaagc | cggcgaacgt | 5800 |
| 5801 | ggcgagaaag | gaagggaaga | aagcgaaagg | agcgggcgct | agggcgctgg | 5850 |
| 5851 | caagtgtagc | ggtcacgctg | cgcgtaacca | ccacacccgc | cgcgcttaat | 5900 |
| 5901 | gcgccgctac | agggcgcgtc | ccattcgcca | 5930 |
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pET-42a(+)
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