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- 2025年11月21日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-44b(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号:
质粒名称: pET-44b(+)
其他名称:
质粒大小（bp）: 7310
抗性: Ampicillin (氨苄 青 霉 素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: 高
蛋白标签: C-HSV, N-His, C-His, N-Thrombin, N-Nus, N-EK
5′ 测序引物序列: T7: 5′-TAATACGACTCACTATAGGG-3′
3′ 测序引物序列: ColiDOWN: 5′-TTCACTTCTGAGTTCGGCATG-3′
GenBank号:
其他属性: Both Nterm and Cterm His tags; Nterm thrombin cleavage site; Nterm enterokinase cleavage site; a,b,c vary by MCS.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_Terminal_primer | 69 | 87 | |
| rrnB_T2_terminator | 168 | 141 | |
| rrnB_T1_terminator | 343 | 300 | |
| rrnB_terminator | 466 | 309 | |
| EK | 694 | 680 | |
| S15 | 790 | 746 | |
| 6xHIS | 2341 | 2324 | |
| T7_transl_en_RBS | 2378 | 2362 | |
| lacO | 2423 | 2396 | |
| T7_promoter | 2441 | 2423 | |
| tet (300 – 563) | 2477 | 2740 | |
| pBRrevBam_primer | 2548 | 2529 | |
| lacI | 2823 | 3914 | |
| ROP | 4486 | 4677 | |
| pGEX_3_primer | 4693 | 4671 | |
| pBR322_origin | 5711 | 5092 | |
| AmpR_promoter | 5798 | 5826 | |
| Ampicillin | 5869 | 6729 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 2353 | 509 | |
| ORF frame 2 | 803 | 2131 | |
| ORF frame 2 | 2324 | 3082 | |
| ORF frame 3 | 2955 | 3914 | |
| ORF frame 1 | 5869 | 6729 |
| Enzyme Name | Cut |
|---|---|
| PacI | 503 |
| XhoI | 530 |
| PmlI | 576 |
| NotI | 592 |
| EagI | 592 |
| HindIII | 599 |
| KpnI | 624 |
| SalI | 626 |
| PstI | 639 |
| AscI | 641 |
| EcoRI | 654 |
| BamHI | 660 |
| SacI | 672 |
| SmaI | 720 |
| XmaI | 718 |
| SacII | 796 |
| SpeI | 827 |
| BglII | 942 |
| MscI | 1074 |
| StuI | 1127 |
| NcoI | 1402 |
| NdeI | 2352 |
| XbaI | 2390 |
| ApaI | 3393 |
| HpaI | 3688 |
| FspI | 6433 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggata | catatttgaa | 100 |
| 101 | tgtatttaga | aaaataaaca | aaagagtttg | tagaaacgca | aaaaggccat | 150 |
| 151 | ccgtcaggat | ggccttctgc | ttaatttgat | gcctggcagt | ttatggcggg | 200 |
| 201 | cgtcctgccc | gccaccctcc | gggccgttgc | ttcgcaacgt | tcaaatccgc | 250 |
| 251 | tcccggcgga | tttgtcctac | tcaggagagc | gttcaccgac | aaacaacaga | 300 |
| 301 | taaaacgaaa | ggcccagtct | ttcgactgag | cctttcgttt | tatttgatgc | 350 |
| 351 | ctggcagttc | cctactctcg | catggggaga | ccccacacta | ccatcggcgc | 400 |
| 401 | tacggcgttt | cacttctgag | ttcggcatgg | ggtcaggtgg | gaccaccgcg | 450 |
| 451 | ctactgccgc | caggcaaatt | ctgttttatc | agcctaggaa | cgcccaactt | 500 |
| 501 | aattaacatt | agtggtggtg | gtggtggtgc | tcgagatcct | ctgggtcttc | 550 |
| 551 | aggagcgagt | tctggctggc | ttgcacgtgt | atacagctgt | gcggccgcaa | 600 |
| 601 | gcttcgaacg | cgtatcgatg | gtaccgtcga | cgtcctgcag | gcgcgcctgt | 650 |
| 651 | acagaattcg | gatccacgag | ctccggactc | ttgtcgtcgt | catcaatcgt | 700 |
| 701 | accagaaccc | gcgctgcccc | gggggaccag | accagttggc | ggtggcgagt | 750 |
| 751 | ccatgtgctg | gcgttcaaat | ttcgcagcag | cggtttcttt | acccgcggag | 800 |
| 801 | tgatggtgat | ggtgatgacc | agaaccacta | gtcgcttcgt | caccgaacca | 850 |
| 851 | gcaaatatta | cgggcagcca | taatcagtgc | tccggctttt | tcgtcggtca | 900 |
| 901 | acccttcgat | atcagccaga | tcatcaatgc | cctgttcggc | gagatcttcc | 950 |
| 951 | agcgtacaaa | cgccacgggc | ggccagtttg | aatgccaaat | cacgatctac | 1000 |
| 1001 | cccttcaagg | ttcagcagat | cgtcagccgg | tttgttatca | ccgaggcttt | 1050 |
| 1051 | cttcctgggc | ctgtgcaatg | gtggccagtg | catttttagc | acgctcgcgc | 1100 |
| 1101 | agtgcttcaa | cggtcggctc | atcaaggcct | tcgatttcca | acagctcttt | 1150 |
| 1151 | catcggcaca | taggccaatt | cttccagcgt | cgagaagcct | tcttctacca | 1200 |
| 1201 | gaacagtcgc | gaagtcttcg | tcgatgtcga | gatatttggt | gaaggtgtcg | 1250 |
| 1251 | atcgctgcgt | gcgcttccgc | ctgatgctta | gcttgcaggt | cgtcaacggt | 1300 |
| 1301 | catcacgttg | agttcccaac | cgctcagttg | cgaagccaga | cgcacgttct | 1350 |
| 1351 | gaccgttacg | gccaatcgcc | tgcgccagat | taccggcttc | aacggcgatg | 1400 |
| 1401 | tccatggtgt | gtttatcttc | atccaccacg | atagaagcaa | cgtctgccgg | 1450 |
| 1451 | tgccattgcg | ttaatcacga | actgcgccgg | gttatcatcc | cacaggacga | 1500 |
| 1501 | tatcgatacg | ctcgccaccc | agttcagtag | acaccgcctg | aacacgcgcg | 1550 |
| 1551 | ccacgcatac | ctacgcaagc | acctaccgga | tcgatacgtt | tatcgttggt | 1600 |
| 1601 | tttcaccgcg | attttcgcac | gagaacccgg | atcgcgagcc | gctgctttaa | 1650 |
| 1651 | tttcaatcac | ttcttcgccg | atttctggca | cttcaatacg | gaacagttcg | 1700 |
| 1701 | atcagcattt | ccggcttgga | acgagtgacg | aacagttgcg | cgccacgcgc | 1750 |
| 1751 | ttccgggcga | acggaataga | gcacgccacg | aacgcggtcg | ccagggcgga | 1800 |
| 1801 | agttttcacg | cggcagcata | tcttcgcgca | ggatcacggc | ttcagcgttg | 1850 |
| 1851 | ttgcccagat | ccagagagat | gttgtcgcgg | tttacttttt | tcaccacgcc | 1900 |
| 1901 | ggtgatgatt | tcaccttcgt | gttcacggaa | ctgatcaacc | accatcgcac | 1950 |
| 1951 | gttcggcttc | acgcactttc | tgcacgataa | cctgttttgc | cgtctgggta | 2000 |
| 2001 | gtgatacggt | caaaggtaac | agactcaatc | tgatcttcaa | cgtaatcgcc | 2050 |
| 2051 | caggttcagg | ctttcatctt | cataacgtgc | ggcttcaagg | gtgatttcct | 2100 |
| 2101 | tggtcggctg | ggtgacttca | tcaacaacta | accagcgacg | gaaagtgtca | 2150 |
| 2151 | aaatcaccgc | ttttgcgatc | gatctgtacg | cggacgtcga | tctcttgttc | 2200 |
| 2201 | atattttttc | tttgttgctg | tcgccagcgc | gctttccaat | gcttcgaaaa | 2250 |
| 2251 | tcttctcgcg | aggtagcgcc | ttttcattgg | atacggcttc | aactacagcc | 2300 |
| 2301 | aaaatttctt | tgttcatact | agaatggtga | tggtggtgat | ggctgctgcc | 2350 |
| 2351 | catatgtata | tctccttctt | aaagttaaac | aaaattattt | ctagagggga | 2400 |
| 2401 | attgttatcc | gctcacaatt | cccctatagt | gagtcgtatt | aatttcgcgg | 2450 |
| 2451 | gatcgagatc | gatctcgatc | ctctacgccg | gacgcatcgt | ggccggcatc | 2500 |
| 2501 | accggcgcca | caggtgcggt | tgctggcgcc | tatatcgccg | acatcaccga | 2550 |
| 2551 | tggggaagat | cgggctcgcc | acttcgggct | catgagcgct | tgtttcggcg | 2600 |
| 2601 | tgggtatggt | ggcaggcccc | gtggccgggg | gactgttggg | cgccatctcc | 2650 |
| 2651 | ttgcatgcac | cattccttgc | ggcggcggtg | ctcaacggcc | tcaacctact | 2700 |
| 2701 | actgggctgc | ttcctaatgc | aggagtcgca | taagggagag | cgtcgagatc | 2750 |
| 2751 | ccggacacca | tcgaatggcg | caaaaccttt | cgcggtatgg | catgatagcg | 2800 |
| 2801 | cccggaagag | agtcaattca | gggtggtgaa | tgtgaaacca | gtaacgttat | 2850 |
| 2851 | acgatgtcgc | agagtatgcc | ggtgtctctt | atcagaccgt | ttcccgcgtg | 2900 |
| 2901 | gtgaaccagg | ccagccacgt | ttctgcgaaa | acgcgggaaa | aagtggaagc | 2950 |
| 2951 | ggcgatggcg | gagctgaatt | acattcccaa | ccgcgtggca | caacaactgg | 3000 |
| 3001 | cgggcaaaca | gtcgttgctg | attggcgttg | ccacctccag | tctggccctg | 3050 |
| 3051 | cacgcgccgt | cgcaaattgt | cgcggcgatt | aaatctcgcg | ccgatcaact | 3100 |
| 3101 | gggtgccagc | gtggtggtgt | cgatggtaga | acgaagcggc | gtcgaagcct | 3150 |
| 3151 | gtaaagcggc | ggtgcacaat | cttctcgcgc | aacgcgtcag | tgggctgatc | 3200 |
| 3201 | attaactatc | cgctggatga | ccaggatgcc | attgctgtgg | aagctgcctg | 3250 |
| 3251 | cactaatgtt | ccggcgttat | ttcttgatgt | ctctgaccag | acacccatca | 3300 |
| 3301 | acagtattat | tttctcccat | gaagacggta | cgcgactggg | cgtggagcat | 3350 |
| 3351 | ctggtcgcat | tgggtcacca | gcaaatcgcg | ctgttagcgg | gcccattaag | 3400 |
| 3401 | ttctgtctcg | gcgcgtctgc | gtctggctgg | ctggcataaa | tatctcactc | 3450 |
| 3451 | gcaatcaaat | tcagccgata | gcggaacggg | aaggcgactg | gagtgccatg | 3500 |
| 3501 | tccggttttc | aacaaaccat | gcaaatgctg | aatgagggca | tcgttcccac | 3550 |
| 3551 | tgcgatgctg | gttgccaacg | atcagatggc | gctgggcgca | atgcgcgcca | 3600 |
| 3601 | ttaccgagtc | cgggctgcgc | gttggtgcgg | acatctcggt | agtgggatac | 3650 |
| 3651 | gacgataccg | aagacagctc | atgttatatc | ccgccgttaa | ccaccatcaa | 3700 |
| 3701 | acaggatttt | cgcctgctgg | ggcaaaccag | cgtggaccgc | ttgctgcaac | 3750 |
| 3751 | tctctcaggg | ccaggcggtg | aagggcaatc | agctgttgcc | cgtctcactg | 3800 |
| 3801 | gtgaaaagaa | aaaccaccct | ggcgcccaat | acgcaaaccg | cctctccccg | 3850 |
| 3851 | cgcgttggcc | gattcattaa | tgcagctggc | acgacaggtt | tcccgactgg | 3900 |
| 3901 | aaagcgggca | gtgagcgcaa | cgcaattaat | gtaagttagc | tcactcatta | 3950 |
| 3951 | ggcaccggga | tctcgaccga | tgcccttgag | agccttcaac | ccagtcagct | 4000 |
| 4001 | ccttccggtg | ggcgcggggc | atgactagca | tgatcgtgct | cctgtcgttg | 4050 |
| 4051 | aggacccggc | taggctggcg | gggttgcctt | actggttagc | agaatgaatc | 4100 |
| 4101 | accgatacgc | gagcgaacgt | gaagcgactg | ctgctgcaaa | acgtctgcga | 4150 |
| 4151 | cctgagcaac | aacatgaatg | gtcttcggtt | tccgtgtttc | gtaaagtctg | 4200 |
| 4201 | gaaacgcgga | agtcagcgcc | ctgcaccatt | atgttccgga | tctgcatcgc | 4250 |
| 4251 | aggatgctgc | tggctaccct | gtggaacacc | tacatctgta | ttaacgaagc | 4300 |
| 4301 | gctggcattg | accctgagtg | atttttctct | ggtcccgccg | catccatacc | 4350 |
| 4351 | gccagttgtt | taccctcaca | acgttccagt | aaccgggcat | gttcatcatc | 4400 |
| 4401 | agtaacccgt | atcgtgagca | tcctctctcg | tttcatcggt | atcattaccc | 4450 |
| 4451 | ccatgaacag | aaatccccct | tacacggagg | catcagtgac | caaacaggaa | 4500 |
| 4501 | aaaaccgccc | ttaacatggc | ccgctttatc | agaagccaga | cattaacgct | 4550 |
| 4551 | tctggagaaa | ctcaacgagc | tggacgcgga | tgaacaggca | gacatctgtg | 4600 |
| 4601 | aatcgcttca | cgaccacgct | gatgagcttt | accgcagctg | cctcgcgcgt | 4650 |
| 4651 | ttcggtgatg | acggtgaaaa | cctctgacac | atgcagctcc | cggagacggt | 4700 |
| 4701 | cacagcttgt | ctgtaagcgg | atgccgggag | cagacaagcc | cgtcagggcg | 4750 |
| 4751 | cgtcagcggg | tgttggcggg | tgtcggggcg | cagccatgac | ccagtcacgt | 4800 |
| 4801 | agcgatagcg | gagtgtatac | tggcttaact | atgcggcatc | agagcagatt | 4850 |
| 4851 | gtactgagag | tgcaccatat | atgcggtgtg | aaataccgca | cagatgcgta | 4900 |
| 4901 | aggagaaaat | accgcatcag | gcgctcttcc | gcttcctcgc | tcactgactc | 4950 |
| 4951 | gctgcgctcg | gtcgttcggc | tgcggcgagc | ggtatcagct | cactcaaagg | 5000 |
| 5001 | cggtaatacg | gttatccaca | gaatcagggg | ataacgcagg | aaagaacatg | 5050 |
| 5051 | tgagcaaaag | gccagcaaaa | ggccaggaac | cgtaaaaagg | ccgcgttgct | 5100 |
| 5101 | ggcgtttttc | cataggctcc | gcccccctga | cgagcatcac | aaaaatcgac | 5150 |
| 5151 | gctcaagtca | gaggtggcga | aacccgacag | gactataaag | ataccaggcg | 5200 |
| 5201 | tttccccctg | gaagctccct | cgtgcgctct | cctgttccga | ccctgccgct | 5250 |
| 5251 | taccggatac | ctgtccgcct | ttctcccttc | gggaagcgtg | gcgctttctc | 5300 |
| 5301 | atagctcacg | ctgtaggtat | ctcagttcgg | tgtaggtcgt | tcgctccaag | 5350 |
| 5351 | ctgggctgtg | tgcacgaacc | ccccgttcag | cccgaccgct | gcgccttatc | 5400 |
| 5401 | cggtaactat | cgtcttgagt | ccaacccggt | aagacacgac | ttatcgccac | 5450 |
| 5451 | tggcagcagc | cactggtaac | aggattagca | gagcgaggta | tgtaggcggt | 5500 |
| 5501 | gctacagagt | tcttgaagtg | gtggcctaac | tacggctaca | ctagaaggac | 5550 |
| 5551 | agtatttggt | atctgcgctc | tgctgaagcc | agttaccttc | ggaaaaagag | 5600 |
| 5601 | ttggtagctc | ttgatccggc | aaacaaacca | ccgctggtag | cggtggtttt | 5650 |
| 5651 | tttgtttgca | agcagcagat | tacgcgcaga | aaaaaaggat | ctcaagaaga | 5700 |
| 5701 | tcctttgatc | ttttctacgg | ggtctgacgc | tcagtggaac | gaaaactcac | 5750 |
| 5751 | gttaagggat | tttggtcatg | acctatttgt | ttatttttct | aaatacattc | 5800 |
| 5801 | aaatatgtat | ccgctcatga | gacaataacc | ctgataaatg | cttcaatcat | 5850 |
| 5851 | gattgaaaaa | ggaagagtat | gagtattcaa | catttccgtg | tcgcccttat | 5900 |
| 5901 | tccctttttt | gcggcatttt | gccttcctgt | ttttgctcac | ccagaaacgc | 5950 |
| 5951 | tggtgaaagt | aaaagatgct | gaagatcagt | tgggtgcacg | agtgggttac | 6000 |
| 6001 | atcgaactgg | atctcaacag | cggtaagatc | cttgagagtt | ttcgccccga | 6050 |
| 6051 | agaacgtttt | ccaatgatga | gcacttttaa | agttctgcta | tgtggcgcgg | 6100 |
| 6101 | tattatcccg | tattgacgcc | gggcaagagc | aactcggtcg | ccgcatacac | 6150 |
| 6151 | tattctcaga | atgacttggt | tgagtactca | ccagtcacag | aaaagcatct | 6200 |
| 6201 | tacggatggc | atgacagtaa | gagaattatg | cagtgctgcc | ataaccatga | 6250 |
| 6251 | gtgataacac | tgcggccaac | ttacttctga | caacgatcgg | aggaccgaag | 6300 |
| 6301 | gagctaaccg | cttttttgca | caacatgggg | gatcatgtaa | ctcgccttga | 6350 |
| 6351 | tcgttgggaa | ccggagctga | atgaagccat | accaaacgac | gagcgtgaca | 6400 |
| 6401 | ccacgatgcc | tgtagcaatg | gcaacaacgt | tgcgcaaact | attaactggc | 6450 |
| 6451 | gaactactta | ctctagcttc | ccggcaacaa | ttaatagact | ggatggaggc | 6500 |
| 6501 | ggataaagtt | gcaggaccac | ttctgcgctc | ggcccttccg | gctggctggt | 6550 |
| 6551 | ttattgctga | taaatctgga | gccggtgagc | gtgggtctcg | cggtatcatt | 6600 |
| 6601 | gcagcactgg | ggccagatgg | taagccctcc | cgtatcgtag | ttatctacac | 6650 |
| 6651 | gacggggagt | caggcaacta | tggatgaacg | aaatagacag | atcgctgaga | 6700 |
| 6701 | taggtgcctc | actgattaag | cattggtaac | tgtcagacca | agtttactca | 6750 |
| 6751 | tatatacttt | agattgattt | aaaacttcat | ttttaattta | aaaggatcta | 6800 |
| 6801 | ggtgaagatc | ctttttgata | atctcatgcc | atcgtgccgc | cagaaattgt | 6850 |
| 6851 | aaacgttaat | attttgttaa | aattcgcgtt | aaatttttgt | taaatcagct | 6900 |
| 6901 | cattttttaa | ccaataggcc | gaaatcggca | aaatccctta | taaatcaaaa | 6950 |
| 6951 | gaatagaccg | agatagggtt | gagtgttgtt | ccagtttgga | acaagagtcc | 7000 |
| 7001 | actattaaag | aacgtggact | ccaacgtcaa | agggcgaaaa | accgtctatc | 7050 |
| 7051 | agggcgatgg | cccactacgt | gaaccatcac | cctaatcaag | ttttttgggg | 7100 |
| 7101 | tcgaggtgcc | gtaaagcact | aaatcggaac | cctaaaggga | gcccccgatt | 7150 |
| 7151 | tagagcttga | cggggaaagc | cggcgaacgt | ggcgagaaag | gaagggaaga | 7200 |
| 7201 | aagcgaaagg | agcgggcgct | agggcgctgg | caagtgtagc | ggtcacgctg | 7250 |
| 7251 | cgcgtaacca | ccacacccgc | cgcgcttaat | gcgccgctac | agggcgcgtc | 7300 |
| 7301 | ccattcgcca | 7310 |
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pET-44b(+)
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