万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存:
60
- 英文名:
pYD1
- 保质期:
三年
- 供应商:
再康生物
- 保存条件:
常温
- 规格:
5ug质粒
pYD1载体
基本信息
| 质粒类型: | 酵母表面展示系统 |
|---|---|
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点,限制性内切酶 |
| 载体大小: | 5009 bp (查看载体序列) |
| 5' 测序引物及序列: | pYD1-F: AGTAACGTTTGTCAGTAATTGC |
| 3' 测序引物及序列: | pYD1-R: GTCGATTTTGTTACATCTACAC |
| 载体标签: | C-His |
| 载体抗性: | Ampicillin (氨苄 青 霉 素) |
| 筛选标记: | TRP1 |
| 备注: | 配套酿酒酵母菌株是EBY100. |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| ZK1629 | pYD1 | 5ug质粒 |
¥1000.00 |
质粒图谱
载体描述
pYD1 is a 5.0 kb expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells. Features of this vector allow regulated expression, secretion, and detection of expressed proteins (see pages 15-16 for more information). The vector contains the following elements:
- AGA2 gene from Saccharomyces cerevisiae. This gene encodes one of the subunits of the a-agglutinin receptor. Fusion of the gene of interest to AGA2 allows secretion and display of the protein of interest.
- GAL1 promoter for regulated expression of the AGA2 gene fusion.
- Xpress epitope and V5 epitope for detection of the displayed protein.
- Polyhistidine (6xHis) tag for detection and possible purification on metal chelating resin.
- TRP1 gene for selection in Saccharomyces cerevisiae.
- CEN6/ARS4 for stable, episomal replication in yeast.
- Ampicillin resistance gene and the pUC origin for selection and replication in E. coli.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
pYD1载体
¥1000





