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- 2025年11月20日
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- 文献和实验
- 技术资料
- 库存：
1
- 英文名：
EpiQuik Histone Demethylase (H3-K4 Specific) Activity/Inhibition Assay Kit
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 组蛋白去加急酶(H3-K4)活性/抑制分析试剂盒详细信息：
-
Product Overview
The EpiQuik™ Histone Demethylase (H3-K4 Specific) Activity/Inhibition Assay Kit is a convenient set of tools that allows the experimenter to measure activity/inhibition of H3-K4 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase (H3-K4 Specific) activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
WHY CHOOSE THE EPIQUIK™ HISTONE DEMETHYLASE (H3-K4 SPECIFIC) ACTIVITY/INHIBITION ASSAY KIT?
- Fast procedure, which can be finished within 3 hours.
- Innovative fluorescent assay with no use of radioactivity, extraction, and chromatography.
- Direct measurement of HDM (H3-K4 specific) activity and inhibition and not interfered by thiol-containing chemicals such as DTT, GSH and 2-mercaptoethanol.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
- Simple, reliable, and consistent assay conditions.
组蛋白去加急酶(H3-K4)活性/抑制分析试剂盒Principle & Procedure
The EpiQuik™ Histone Demethylase (H3-K4 specific) Activity/Inhibition Assay Kit is designed for measuring total histone demetylase (H3-K4 Specific) activity/inhibition. In the assay with this kit, the unique di-methylated histone H3-K4 substrate is stably captured on the strip wells. Active HDMs bind to and demethylate histone H3-K4 substrate. The remaining un-demethylated substrate can be recognized with high affinity anti-methylated histone H3-K4 antibody. The ratio or amount of the un-demethylated histone, which is inversely proportional to HDM enzyme activity, can then be fluorimetrically quantified.
SCHEMATIC PROCEDURE: 组蛋白去加急酶(H3-K4)活性/抑制分析试剂盒Product Components
HD1 (10x wash buffer)
HD2 (HDM assay buffer)
HD3 (HDM substrate)*
HD4 (HDM standard, 20 µg/ml)*
HD5 (capture antibody 100 µg/ml)*
HD6 (detection antibody 200 µg/ml)*
HD7 (fluoro-developer)*
HD8 (fluoro enhancer)*
HD9 (fluoro-dilution)
8 well assay strip (with frame)
User guide
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* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.组蛋白去加急酶(H3-K4)活性/抑制分析试剂盒User Guide & MSDS
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文献和实验的作用是调控基因表达。例如组蛋白甲基化多导致基因沉默，去甲基化则相反；乙酰化一般是转录激活，去乙酰化则相反。当然，也可在此基础上产生复杂的生物学效应。例如组蛋白去乙酰化酶 HDAC 可影响免疫系统；H3K4me3、H3K9me2 能够调控记忆的形成, 而且 H3K 甲基化与 X 染色体失活、基因组印记和异染色质形成有关；H3 乙酰化通过多种机制调控以来 ATP 的染色质重塑 ，并参与炎症反应；H2A、H2B 泛素化则与 DNA 损害反应有关；而 H3S28 磷酸化与 H3K27 乙酰化可激活转录
化可以促进转 录激活 (Greer et al., 2012)，而赖氨酸甲基化则同时涉足转录激活和抑制，具体取决于甲基化位点。这 种灵活性可以解释为甲基化不会改变组蛋白电荷，也不会直接影响组蛋白-DNA 相互作用，与乙酰化不同。 赖氨酸可以单甲基化、双甲基化或三甲基化，从而为每个甲基化位点提供进一步的功能多样性。例如， 组蛋白 H3 K4 （H3K4me1 和 H3K4me3）上的单甲基化和三甲基化都是活化标志物，但是也有着特殊 的细微差别：H3K4me1 通常会标记转录增强子，而 H3K4
HDAC（Histone Deacetylase）组蛋白去乙酰化酶活性测定及药物筛选方案
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