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通用Di-Methyl组蛋白H3-K4定量试剂盒(Color

imetric)
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  • 美国eBioscince
  • ZK-0019688
  • 美国/中国
  • 2025年11月20日
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    上海再康生物科技有限公司
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    • 英文名：

      EpiQuik Global Di-Methyl Histone H3-K4 Quantification Kit (Colorimetric)

    • 保质期：

      6个月

    • 供应商：

      上海再康

    • 保存条件：

      4℃保存

    • 通用Di-Methyl组蛋白H3-K4定量试剂盒(Colorimetric) 详细信息：
    • Product Overview

      The EpiQuik™ Global Di-Methyl Histone H3-K4 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global di-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

      WHY CHOOSE THE EPIQUIK™ GLOBAL DI-METHYL HISTONE H3-K4 QUANTIFICATION KIT (COLORIMETRIC)?
      • Quick and efficient, which can be finished within 2.5 hours.
      • Innovative colorimetric assay without need for radioactivity, electrophoresis, and chromatography.
      • Specifically capturing di-methylated H3-K4 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
      • The control is conveniently included for quantification of the amount of di-methylated H3-K4.
      • Strip microplate format makes the assay flexible: manual or high throughput.
      • Simple, reliable, and consistent assay conditions.
      通用Di-Methyl组蛋白H3-K4定量试剂盒(Colorimetric) Principle & Procedure


      The EpiQuik™ Global Di-Methyl Histone H3-K4 Quantification Kit (Colorimetric) is designed for measuring global histone H3-K4 di-methylation. In an assay with this kit, the di-methylated histone H3 at lysine 4 is captured to the strip wells coated with anti-dimethyl H3-K4 antibody. The captured di-methylated histone H3-K4 can be then detected with a labeled detection antibody followed by color development reagent. The ratio of di-methylated H3-K4 is proportional to the intensity of absorbance. The absolute amount of di-methylated H3-K4 can be quantitated by comparing to the standard control.

      SCHEMATIC PROCEDURE:

      通用Di-Methyl组蛋白H3-K4定量试剂盒(Colorimetric) Product Components

      C1 (10 x wash buffer)
      C2 (antibody buffer)
      C3 (detection antibody, 1 mg/ml)*
      C4 (color developer)
      C5 (stop solution)
      Standard control (100 µg/ml)*
      8 well sample strips (with frame)
      8 well standard control strips
      User guide
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      * For maximum recovery of the products, centrifuge the original vial prior to opening the cap.
      通用Di-Methyl组蛋白H3-K4定量试剂盒(Colorimetric) User Guide & MSDS

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