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- ZK-0019655
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- 2025年11月20日
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- 详细信息
- 技术资料
- 库存：
1
- 英文名：
EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27)
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 组蛋白甲基转移酶活性/抑制分析试剂盒（H3-K27)详细信息：
-
Product Overview
The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is a convenient set of tools that allows the experimenter to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27. The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition experiment without the need for radioactivity or any special equipment.
WHY CHOOSE THE EPIQUIK™ HISTONE METHYLTRANSFERASE ACTIVITY/INHIBITION ASSAY KIT (H3-K27)?- Very rapid procedure, which can be completed within 3 hours.
- Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
- Specific measurement of activity/inhibition of H3-K27 histone methyltransferases.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
- Extremely simple, reliable, and consistent assay conditions.
组蛋白甲基转移酶活性/抑制分析试剂盒（H3-K27)Principle & Procedure
The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is designed for measuring HMTs that specifically target histone H3 at lysine 27. In an assay with this kit, the histone substrate is stably captured on the strip wells through biotin-streptavidin binding. HMT enzymes transfer methyl group to histone H3 substrate from Adomet to methylate the substrate at lysine 4. The methylated histone H3-K27 can be recognized with high-affinity antibody. The ratio or amount of methylated H3-K27, which is directly proportional to enzyme activity, can be quantified through HRP conjugated secondary antibody-color development system. The HMT activity is then calculated based on the amount of methylated H3-K27 converted by the HMTs.
SCHEMATIC PROCEDURE: 组蛋白甲基转移酶活性/抑制分析试剂盒（H3-K27)Product Components
HE1 (10x wash buffer)
HE2 (histone assay buffer)
HE3 (Adomet)*
HE4 (biotinylated substrate, 25 µg/ml)*
HE5 (HMT standard, 10 µg/ml)*
HE6 (capture antibody, 100 µg/ml)*
HE7 (detection antibody, 100 µg/ml)*
HE8 (developing solution)
HE9 (stop solution)
Control enzyme (150 µg/ml)*
8 well assay strips (with frame)
User guide
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zkA004Hu 血管紧张素转化酶(AHZ)ELISA试剂盒 检测范围
zkA005Hu 血管紧张素Ⅱ(ANGⅡ)ELISA试剂盒 检测范围
zkA006Hu 双调蛋白(AREG)ELISA试剂盒 检测范围
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zkA008Hu 血管生成素1(ANGPT1)ELISA试剂盒 检测范围
zkA009Hu 血管生成素2(ANGPT2)ELISA试剂盒 检测范围
zkA011Hu 血管生成素2(ANGPT2)ELISA试剂盒 检测范围
zkA112Hu β细胞素(βTC)ELISA试剂盒 检测范围
zkA013Hu 骨成型蛋白2(BMP2)ELISA试剂盒 检测范围
zkA014Hu 骨成型蛋白4(BMP4)ELISA试剂盒 检测范围
zkA015Hu 骨成型蛋白受体1A(BMPR1A)ELISA试剂盒 检测范围
zkA017Hu 上皮性钙黏附蛋白(CDHE)ELISA试剂盒 检测范围
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zkA024Hu 内分泌腺来源血管内皮生长因子(EG-VEGF)ELISA试剂盒 检测范围
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* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.组蛋白甲基转移酶活性/抑制分析试剂盒（H3-K27)User Guide & MSDS -
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