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- 美国eBioscince
- ZK-0019545
- 美国/中国
- 2025年11月20日
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- 详细信息
- 技术资料
- 库存：
1
- 英文名：
ultra Methylamp Global DNA Methylation Quantification Ul
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 通用DNA甲基化定量试剂盒详细信息：
-
Product Overview
Product FlyerThe Methylamp™ Global DNA Methylation Quantification Ultra Kit is an improved version of the popular Methylamp™ Global DNA Methylation Quantification Kit. As a complete set of essential components, the kit enables the experimenter to quantify global DNA methylation through an ELISA-like reaction using Epigenteks uniquely simplified and streamlined procedure. The entire procedure can be completed in less than 4 hours in either manual or high throughput analysis. In addition to having a shorter procedure, the improved kit also has higher sensitivty than its predecessor. It is suitable for detecting global DNA methylation status using genomic DNA isolated from variety of cultured cells, fresh and frozen tissue, paraffin-embedded tissue, plasma/serum sample, and body fluid sample, etc. The universal positive control included in the kit allows for quantitation of methylated DNA from any species.
WHY CHOOSE THE METHYLAMP™ GLOBAL DNA METHYLATION QUANTIFICATION KIT?
- Colorimetric quantification without radioactivity, extraction, and chromatography.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
通用DNA甲基化定量试剂盒IMPROVEMENTS OVER ITS PREDECESSOR:- Shorter procedure, which can be finished in less than 4 hours.
- Significantly higher sensitivity -- detection limitation can be as low as 0.2 ng of methylated DNA.
- Universal positive control suitable for quantifying methylated DNA from any species.
Principle & Procedure
The Methylamp™ Global DNA Methylation Quantification Ultra Kit contains all reagents required for quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with DNA affinity substance. The methylated fraction of DNA can be recognized by 5-methylcytosine antibody and quantified through an ELISA-like reaction. The amount of methylated DNA is proportional to the OD intensity.
SCHEMATIC PROCEDURE:
Quantification of Methylated DNA.通用DNA甲基化定量试剂盒Product Components
GU1 (10 X wash buffer)
GU2 (DNA binding solution)
GU3 (positive control,100 µg/ml)**
GU4 (block solution)
GU5 (capture antibody, 1000 µg/ml)*
GU6 (detecting antibody, 400 µg/ml)*
GU7 (enhance solution)
GU8 (developing solution)
GU9 (stop solution)
8 well assay strips (with frame)
User guide
60707-50 细菌DNAout
60707-250 细菌DNAout
60802-50 柱式细菌DNAout
80927A-4 大提柱式细菌DNAout
80927B-4 大提柱式细菌DNAout
60806-50 一步式细菌DNAout
70702-50 分枝杆菌DNAout
70703-50 柱式分枝杆菌DNAout
60701-30 土壤DNAout
60701-100次 土壤DNAout
71205-50 柱式土壤DNAout
80928-4 大提柱式土壤DNAout
3674-50 一管式病毒DNAout
3674-200 一管式病毒DNAout
70701-50 柱式病毒DNAout
80921-1 96孔板病毒DNAout
80921-5 96孔板病毒DNAout
80101-50 一站式病毒DNA-RNAout
70202-50 柱式酵母质粒DNAout
90607-50 柱式BAC DNAout
90608-5 中提柱式BAC DNAout
60205-50 柱式质粒DNAout
60205-100 柱式质粒DNAout
80912-5 经典法大提质粒DNAout
80912-10 经典法大提质粒DNAout
80916-1 96孔板经典法质粒DNAout
70903-50 一步式质粒DNAout2.0
70903-100 一步式质粒DNAout2.0
80917-1 一步法96孔板质粒DNAout
70804-50 单菌落质粒DNAout
80932-1 96孔板单菌落质粒DNAout
80932-5 96孔板单菌落质粒DNAout
71101-5 一步法大提质粒DNAout
60807-50 经典法无内毒素质粒DNAout
81107-5 经典法无内毒素大提质粒DNAout
* Spin the solution down to the bottom before use.
** This control is synthesized polynucleiotide methylated at every 5-cytosine通用DNA甲基化定量试剂盒User Guide & MSDS
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通用DNA甲基化定量试剂盒
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