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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 英文名:
Skimmed Milk Powder
- 保质期:
2 年
- 供应商:
上海再康生物科技有限公司
- 保存条件:
室温保存,保质期 2 年。常温运输。
- 规格:
50g;100g;250g;500g
产品简介
脱脂奶粉可用于制备微生物培养基;配制ELISA、western等实验的封闭液。western blot经过转印以后,膜上有了蛋白,但是还有很多地方是没有蛋白覆盖的,通过脱脂奶粉的蛋白孵育后,这些空白的膜都被脱脂奶粉的蛋白覆盖了,就防止后续孵育的一抗和二抗与印迹膜的非特异结合。在科研实验中,脱脂奶粉也是常用的生化试剂之一。
Fat-free powdered milk is used as a saturating agent to block nonspecific binding sites on supports like blotting membranes (nitrocellulose, polyvinylidene fluoride (PVDF) or nylon), preventing binding of further detection reagents and subsequent background. It may be referred as Blotto. The major protein of milk, casein, is responsible for most of the binding site saturation effect.
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文献和实验Estimation of Fat in Milk Samples
deficiencies. Principle The fat can be separated from fat-containing milk/milk powder through the addition of sulphuric acid. The separation is made by using amyl alcohol and centrifugation. The fat content is read directly on a special calibrated
, like milk powder, fetal calf serum (FCS), or fish extracts, to this day. A lot of university research is still conducted using these blocking reagents, as witnessed by many, otherwise high quality research articles. With these reagents, elevated
Freeze-Drying Fungi Using a Shelf-Freeze Drier
the use of standard shelf freeze-drying and skimmed milk as a suspending solution/lyoprotectant. This approach has been employed to freeze-dry the majority of the 50,000 fungal strains that have been successfully lyophilized
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