Leukemia Inhibitory Factor, re

Leukemia Inhibitory Factor, recombinant mouse 保存于运输说明：
Maintain at 2-8°C for 6 - 12 months. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added.
详细信息：
Leukemia Inhibitory Factor, recombinant mouse 重组小鼠白血病抑制因子


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Description: Leukemia Inhibitory Factor, recombinant mouse


Trade Name: Chemicon (Millipore)

Product Overview: Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is a 20 kDa protein containing 181 amino acid residues.

Leukemia Inhibitory Factor, recombinant mouseKey Applications:
Stem Cell Culture
Cell Culture

Application Notes: RECOMMENDED QC PROTOCOL

M1 Bioassay

1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.

2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.

3. Add 100 μL of 5% FCS in isotonic saline to two control slides.

4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.

5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).

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Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.

Leukemia Inhibitory Factor, recombinant mouseUsage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Species: Mouse

Stem Cell Type:
Mouse Embryonic Stem Cells
Neural Stem Cells
Hematopoietic Stem Cells
Mesenchymal Stem Cells
Induced Pluripotent Stem Cells

Stem Cell Workflow Stage:
Maintenance & Expansion
Differentiation
General Cell Culture

Source: mLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.

Presentation: Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.

Storage Conditions: Maintain at 2-8°C for 6 - 12 months. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added.
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.

Specific Activity:
The activity of mouse LIF is determined by the ability to induce differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(E8) units/mg, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.

Cell Culture Reagent Type: Cytokines & Growth Factors

Protein or Enzyme Type: Cytokines & Growth Factors

Alternate Names: LIF; Differentiation-stimulating Factor; D Factor

Concentration: 10 μg/mL

Purity: Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.
Leukemia Inhibitory Factor, recombinant mouse
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