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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海再康生物科技有限公司
- 库存:
50
- 克隆性:
多克隆
- 保质期:
12个月
- 抗体英文名:
1.5M Tris-HCL Buffer, pH8.8
- 保存条件:
常温
- 规格:
100mL;500mL
产品简介
用于配制SDS-PAGE或native PAGE的下层胶,进口试剂配制。
Tris也称Tris base或2-Amino-2-(hydrozkmethyl)-1,3-propanediol。分子式为C4H11NO3,分子量121.14,纯度>99.9%。
注意事项
1)对人体有刺激性,请注意适当防护。
2)为了您的安全和健康,请穿实验服并戴一次性手套操作。
Tris, or tris(hydrozkmethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids.
"Tris" is a chemical compound used as a buffer. The full name is tris(hydrozkmethyl)aminomethane. Tris has the ability to absorb counter ions (+H and -OH) so as to help keep the solution that they are in at a stable pH level. When the pH of Tris is set using HCl (h y d r o c h l o r i c a c i d) the buffer is called Tris HCl.
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文献和实验0.5M EDTA FW M L pH8.0 xg = 292.25 0.5 0.5 **93.06g** Add to about 300mls H2 O while spinning
【求助】着急,不知道10mmol/L的Tris-HCl(pH8.0)如何配制?
ilovelc999 很着急,不知道10mmol/L的Tris-HCl(pH8.0)如何配制?有哪位知道吗?麻烦告诉我一下好吗。 mybbff 先配成10mM的,水先不要加足,调节pH到8.0后再定容到预定体积。 ilovelc999 恩,知道啦,多谢版主! wangbingying66 1.称取1.211g Tris 2.加800ml去离子
Preparation of Microinjection Buffer
Buffer composition is 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 30 microM spermine, 70 microM spermidine, 100 mM NaCl. This buffer is more likely to produce transgenic mice with intact, unfragmented DNA molecules. See the following references: Schedl
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