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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
再康生物
- 样本:
血清液/组织/尿
- 库存:
大量
- 标记物:
见 说 明 书
- 适应物种:
Human/Mouse/Rat
- 应用:
科学研究
- 检测方法:
Western blot/Elisa/IHC
- 检测范围:
见 说 明 书
- 规格:
100 ug
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文献和实验Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).
using Dynabeads® CD3⁄CD28 CTS™. Following separation, the CD3 T cells are cultured in the presence of the beads. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads®, the beads will provide both the primary and co-stimulatory signals
-20 in 50 mM Na-phosphate buffer, pH 7.3. Goat-anti-mouse antibodies labeled with AlexaFluor-488 were from Invitrogen, Inc. (dye/protein ratio 7). Microscope glass slides, 3 × 1 in., and 1 mm thick, were from VWR. Water was purified by Milli-Q
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