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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
再康生物
- 样本:
血清液/组织/尿
- 库存:
大量
- 标记物:
见 说 明 书
- 适应物种:
Human/Mouse/Rat
- 应用:
科学研究
- 检测方法:
Western blot/Elisa/IHC
- 检测范围:
见 说 明 书
- 规格:
100 ug
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文献和实验. Test each tube w/H2O and discard.Add protein mixture to dialysis tube. Stir slowly in 100 mM Tris buffer or Binding buffer until pink color is out.Transfer protein mixture (Ab) to 50 ml Falcon tubes to determine volume.Dilute the mixture
Sup. 4L 100 mM Tris/pH7.8 or Binding Buffer BD tubing 14-170-12F (Fisher) Polyethylene tubing Clay Adanes. 18 Gauge needles. Binding Buffer (BioRad): make 1L. 314g/L ddH2 O and filter through 0.22 um, filter. pH=9.0
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
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