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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
再康生物
- 样本:
血清液/组织/尿
- 库存:
大量
- 标记物:
见 说 明 书
- 适应物种:
Human/Mouse/Rat
- 应用:
科学研究
- 检测方法:
Western blot/Elisa/IHC
- 检测范围:
见 说 明 书
- 规格:
100 ug
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文献和实验nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin
Biotin labeling and hybridization of probe to chromosomes
Biotin labeling and hybridization of probe to chromosomes Suggested chemicals: Nick Translation Kit, Enzo Diagnostics Cat. No. 42803. Biotin labeling of plasmid or genomic DNA. Remove slides
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
in PBS with 100 U/ml RNase. Analyze on any 488 nm argon- 630 nm HeNe or diode dual laser flow cytometer for PI and Cy5 fluorescence. Both PI and Cy5 should be analyzed in linear mode. Recipes Anti-cyclin D1 (Pharmingen cat. no. 14561A, clone G12
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