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- ZK-0106256
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- 2025年11月20日
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- 库存：
大量
- 保质期：
12个月
- 供应商：
再康生物
- 保存条件：
20℃
- 规格：
25 ug
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
Immunofluorescent Staining of Mouse and Rat Leukocytes
. Gently vortex immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 15 min. Centrifuge 200 x g for 5 min. Carefully aspirate and dispose of supernatant, without disturbing the pellet. Resuspend pellet in 1X
Cell Surface Immunofluorescence Staining Protocol
16/32 on ice for 10 minutes. In the absence of an effective/available blocking antibody for human and/or rat Fc receptors, an alternative approach is to pre-block cells with excess irrelevant purified Ig from the same species and same isotype
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