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文献和实验Lectin-Induced Calcium Mobilization in Human Platelets: Use of Fluorescent Probes
, such as glycoprotein IIb–IIIa complex (GPIIb–IIIa) in controlling calcium homeostasis in platelets. GPIIb–IIIa is the platelet receptor for fibrinogen and other adhesive proteins and belongs to the integrin superfamily (4 ). It represents a high affinity binding site
Detection of High-Affinity 4-Integrin Upon Leukocyte Stimulation by Chemoattractants or Chemokines
(7 ). In this protocol, binding of recombinant chimeric human VCAM-1 is used to identify high-affinity α4-integrins by flow cytometry. In addition, a recently reported method for monitoring α4-integrin affinity in real time using a ligand-mimetic peptide, which contains
, this expression and functional system has been used to show that the preincubation of Human Odorant Binding Protein 2A decrease the calcium response of OR 17-210 following stimulation by acetophenone and beta ionone.
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