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文献和实验Generation of Monoclonal Antibodies to Native Active Human Glycosyltransferases
Complex carbohydrates serve a wide range of biological functions in cells and tissues. Their biosynthesis involves more than 200 distinct glycosyltransferases in human cells, and the expression, properties, and topology of these enzymes
Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase
In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification
7 ), with which they exhibit a high degree of amino acid sequence conservation (7 –9 ). Considering the roles served by E. coli SSB in bacterial replication in helix destabilization (10 ) and in enhancing DNA polymerase processivity (11 –12 ) and fidelity (13 –14
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