- 询价
- ZK-Bioscience
- ZK-0105076
- 中国/美国/德国
- 2025年11月20日
- WB, ICC, IHC-P, IHC-F, ELISA
- Rabbit，兔
- 见 说 明 书
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
见 说 明 书
- 克隆性：
多克隆
- 抗体英文名：
Polyclonal Antibody to DNA Methyltransferase 1 (DNMT1)
- 抗体名：
DNA甲基转移酶1(DNMT1)多克隆抗体
- 标记物：
未标记
- 宿主：
Rabbit，兔
- 适应物种：
见 说 明 书
- 免疫原：
见 说 明 书
- 亚型：
见 说 明 书
- 形态：
液态/粉末
- 应用范围：
WB, ICC, IHC-P, IHC-F, ELISA
- 浓度：
200ug/ml
- 保存条件：
-20oC
| Organism species | Homo sapiens (Human) |
| Product No. | ZKJ244Hu01 |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunoglobulin Type | IgG |
| Purification | Affinity Chromatography |
| Applications | WB, ICC, IHC-P, IHC-F, ELISA |
| Concentration | 200ug/ml |
| UOM | 100ug |
| Conjugate | No Conjugate |
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.
containing 0.02% NaN3, 50% glycerol.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
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文献和实验A method for purification, identification and validation of DNMT1 mRNA binding proteins
DNA methyltransferase 1 (DNMT1) is the enzyme responsible for the maintenance of DNA methylation patterns during cell division. DNMT1 expression is tightly regulated within the cell cycle. Our previous study showed that the binding of a protein
、癌变等生命现象有着重大关系。二、 DNMT（DNA甲基转移酶）对催化DNA 甲基化的DNA 甲基转移酶（DNAmethyltransferase, Dnmt）的研究可以揭示DNA 甲基化对基因表达调控的机制，从而研究与之相关的重要生命活动。DNA 甲基转移酶有两种: 1) DNM T1, 持续性DNA 甲基转移酶—— 作用于仅有一条链甲基化的DNA 双链, 使其完全甲基化, 可参与DNA 复制双链中的新合成链的甲基化,DNMT1 可能直接与HDAC (组蛋白去乙酰基转移酶) 联合作用阻断转录
Inhibition of DNA Methylation in Somatic Cells
DNA methylation plays a significant role in the expression of the genetic code and affects early growth and development through its influence on gene expression. DNA methyltransferase 1 (Dnmt1) is the enzyme responsible for maintaining
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