src原癌基因抗体

src原癌基因抗体产品介绍

src原癌基因具有酪氨酸激酶活性，参与调节正常细胞的生长与分化，在控制细胞增殖的信息转导途径中起作用；src原癌基因同样有活化癌基因的作用，能够诱导细胞的异常增殖和导致肿瘤发生。
c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
mol wt:60kDa
Other Aliases:
ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specific protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000030931; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60Src; pp60c src; pp60csrc; Proto oncogene tyrosine protein kinase Src; Protooncogene SRC; protooncogene SRC Rous sarcoma; SRC Oncogene; SRC1; Tyrosine kinase pp60c src; Tyrosine protein kinase SRC1; v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian; vsrc avian sarcoma (Schmidt Ruppin A2) viral oncogene homologzk-15214R C6orf1156号染色体开放阅读框115抗体
zk-15215R C6orf1236号染色体开放阅读框123抗体
zk-15216R C6orf129 6号染色体开放阅读框129抗体
zk-15217R C6orf1306号染色体开放阅读框129抗体
zk-15218R C6orf1326号染色体开放阅读框132抗体
zk-10342R Phospho-Calcineurin B (Tyr106)磷酸化钙调磷酸酶B亚基B1抗体
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zk-15197R C5 补体C5抗体
zk-15201R C5orf245号染色体开放阅读框24抗体
zk-15204R C5ORF425号染色体开放阅读框42抗体
zk-15208R C5orf495号染色体开放阅读框49抗体
zk-15203R C5orf355号染色体开放阅读框35抗体
zk-13856R CEP192中心体蛋白192抗体
zk-13857R CEP27中心体蛋白27抗体
zk-13858R CEP290中心体蛋白290抗体
zk-13859R CEP63中心体蛋白63抗体
zk-13860R CEP70中心体蛋白70/p10结合蛋白抗体
zk-13861R CEP72中心体蛋白72抗体
zk-13865R CEP95中心体蛋白95抗体
zk-13864R CEP89中心体蛋白89抗体
zk-13866R CERD4CERD4蛋白抗体
zk-13867R CES1P1CES1P1蛋白抗体
zk-13868R CES2羧酸酯酶2抗体
zk-13869R CES3羧酸酯酶3抗体
zk-13870R CES4A羧酸酯酶4A抗体
zk-13871R CES6羧酸酯酶6抗体
zk-13872R CezanneCezanne蛋白抗体
zk-13873R CFC1内脏移位线管蛋白CFC1蛋白抗体
zk-13874R CFHL2补体因子H相关蛋白2抗体
src原癌基因抗体