- $395
- innov-research
- ZK-0043220
- 美国/英国
- 2025年11月20日
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- 供应商：
再康生物
- 库存：
50
- 规格：
96 tests
Dog IgE ELISA Kit
Catalog No.: IDGIGEKT
$395
Category: Assay and ELISA Kits
Product Description
Dog IgE ELISA Kit
Dog IgE ELISA Kit are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in dog biological samples. In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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文献和实验Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE
to anaphylaxis. Here, we describe the history behind the IgE discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is followed by our ELISA protocol for mouse IgE detection.
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
大鼠 免疫球蛋白 E(IgE) 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本中 免疫球蛋白E(IgE) 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 大鼠 免疫球蛋白 E(IgE) 水平。用纯化的大鼠 IgE 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 免疫球蛋白 E(IgE
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