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再康生物
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50
- 规格：
96 tests
Human Protein S ELISA Kit
Catalog No.: IRKTAH2605
$495
Category: Assay and ELISA Kits
Product Description
Human Protein S ELISA Kit
Human Protein S ELISA Kit is designed for detection of human protein s in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human protein s in less than 3 hours. A polyclonal antibody specific for human protein s has been pre-coated onto a 96-well microplate with removable strips. Protein S in standards and samples is competed by a biotinylated protein s sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Protein S is a single-chain vitamin K-dependent plasma glycoprotein consisting of 635 amino acids with a molecular weight of about 70 kDa (1 - 3). It functions as a cofactor for the anticoagulant activated protein c to inhibit blood coagulation. In plasma, it is both in a free cofactor active form and in an inactive form complexed with C4b-binding protein (C4BP) (4). The complex acts as a bridge between coagulation and inflammation due to the involvement of C4BP in regulating complement activation. Hereditary protein s deficiency causes recurrent venous thrombosis (5). Acquired protein s deficiency is associated with nephrotic syndrome, disseminated intravascular coagulation, liver disease and the use of oral anticoagulants, leading to increased thrombotic risk (6).
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文献和实验the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
Analysis of the Protein S Gene in Protein S Deficiency
Protein S (PS) is a 71-kDa vitamin K-dependent glycoprotein first identified in human plasma by DiScipio and colleagues in 1977 (1 ), a year after the discovery of the anticoagulant protein C (PC) (2 ,3 ). A few years later, Walker
part of QIAGEN's solution for preparing multiple analytes from the same sample. These include Allprotect Tissue Reagent, which stabilizes DNA, RNA, and protein in tissues, and RNAprotect Cell Reagent, which stabilizes DNA and RNA in cells. Both reagents deliver
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