Human tPA Total ELISA Kit

Human tPA Total ELISA Kit

Catalog No.: IHTPAKT-TOT

$495
Category: Assay and ELISA Kits

Product Description

Human tPA Total ELISA Kit

Human tPA Total ELISA Kit is used for the sensitive measurement of total human tPA antigen is easily performed with this Human tPA Total ELISA Kit. tPA binds to the capture antibody coated on the microtiter plate. Free, latent and complexed tPA will bind to the capture antibody.

Functionally active tPA will form a covalent complex with biotinylated human PAI-1 which is bound to the avidin coated on the microtiter plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, polyclonal anti-human tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound polyclonal antibody is reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human tPA. The amount of color development is directly proportional to the concentration of active tPA in the sample.

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