Human uPA ELISA Kit

Human uPA ELISA Kit

Catalog No.: IHUPAKT

$495
Category: Assay and ELISA Kits

Product Description

Human uPA ELISA Kit

The sensitive measurement of functionally active human uPA is easily performed with Human uPA ELISA Kit. Active uPA binds to functionally active PAI-1 coated on the microtiter plate (patent pending). Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected.Functionally active uPA present in plasma will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Latent or complexed uPA will not bind to the plate and will not be detected. Unbound uPA samples are washed away and an anti-uPA primary antibody is added. Excess primary antibody is washed away and bound antibody is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active uPA in the sample.

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