Human IgA ELISA Kit

Human IgA ELISA Kit

Catalog No.: IHUIGAKT

$325
Category: Assay and ELISA Kits

Product Description

Human IgA ELISA Kit

Human Immunoglobulin A (IgA) antigen assay is intended for the quantitative determination of total human IgA antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgA subclasses.

Human IgA will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgA antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human IgA. Color development is directly proportional to the concentration of total IgA in the samples.

Human IgA standard provided is calibrated against the WHO International Standard for Immunoglobulins G, A and M, Human Serum distributed by NIBSC (67/086), South Mimms, Potters Bar, Hertfordshire, UK.

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