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- 详细信息
- 技术资料
- 靶点:
来电咨询
- 克隆性:
多克隆
- 标记物:
见 说 明 书
- 宿主:
Goat,Rabbit,Mouse
- 适应物种:
人,大鼠,小鼠,兔
- 形态:
液态/粉状
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 保存条件:
-20℃保存
- Retrieve-All Antigen Unmasking System 3: Acidic, 100x (SIGNET)保存于运输说明:
- Store at room temperature.
- 详细信息:
-
Description Retrieve-All Antigen Unmasking System 3: Acidic (pH 4.8); 100x
(Formerly Signet Catalog No.: 1900)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form Antigen Unmasking Solution Specificity Retrieve-All Antigen Unmasking System 3: Acidic, 100x (SIGNET)Retrieve-All is an antigen un-masking solution available in the following three pH formulas for use in heat induced un-masking procedures:
Retrieve-All 1 (Universal pH 8)
Retrieve-All 2 (Basic pH 10)
Retrieve-All 3 (Acidic pH 4.8)Suggested Working Dilution The optimal antigen unmasking condition is a function of temperature (90 to 100°C) and incubation time (5-30 minutes) and should be established by each investigator.
Protocol
1. Deparaffinize sections, rehydrate through graded alcohols, DI water, into PBS for 5 min.
2. Preheat Retrieve-All buffer to 92°C*.
3. Place slides into the preheated Retrieve-All buffer.
4. Incubate for 10 minutes at 92°C.
Retrieve-All Antigen Unmasking System 3: Acidic, 100x (SIGNET)5. Remove the container with slides and let it cool at room temperature for 5-10 minutes.
6. Rinse in distilled water or PBS.
7. Proceed with usual immunohistochemical staining.Notes * Concentrated buffers (100x) may show minimal precipitation after long term storage without any effect to HIER Storage Store at room temperature. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Retrieve-All Antigen Unmasking System 3: Acidic, 100x (SIGNET)
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Retrieve-All Antigen Unmasking System 3: Acidic, 100x (SIGNET)
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