- 询价
- R&D
- ZK-0102225
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- Cytokeratin 20 (Ks 20.8) Monoclonal Antibody详细信息：
-
Description Monoclonal Antibody against Human Cytokeratin 20 Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone Ks 20.8 Form Purified Antibody (in 0.05M Tris/HCL, 0.015M sodium azide, 1% BSA, pH 7.5) Host Mouse Species Reactivity Human IsoType Cytokeratin 20 (Ks 20.8) Monoclonal Antibody IgG2a Specificity CK 20 belongs to the intermediate filament proteins, which create a cytoskeleton in almost all cells. In contrast to other intermediate filaments, cytokeratins (CKs) are made up of a highly complex multigene family of 40 to 68 kDa polypeptides. 20 distinct CK polypeptides have been revealed in various human epithelial cells and their malignant counterparts. They can be divided into an acidic (type I) and a neutral-basic (type II) subfamily. CK 20, a 46 kDa protein, is less acidic than the other type I cytokeratins. CK 20 positivity (5-100% CK-20-positive tumour cells) has been reported in the vast majority of adenocarcinomas of the colon, mucinous ovarian tumours, transitional-cell and Merkel cell carcinomas, and frequently also in adenocarcinomas of the stomach (50%), bile system (75%), and pancreas (40%). Less than 2% of breast and lung adenocarcinomas and squamous cell carcinomas were positive, while adenocarcinomas of the ovary (non-mucinous) and endometrium, and renal cell and small cell carcinomas showed only scattered positive cells in some cases.
Anti-Human CK 20, Clone Ks 20.8, labels a 46 kDa polypeptide corresponding to CK 20 in Western immunoblotting of SDS-PAGE separated cytoskeletal proteins from human duodenal mucosa.
Cells labelled by the antibody display a cytoplasmic staining pattern
This antibody was generated agains CK20 isolated by SDS-PAGE from cytoskeletal material obtained from human duodenal mucosa.Uses This antibody is effective in immunoblotting, immunocytochemistry (ICC) and immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:25 - 1:50*
- IHC: 1:25 - 1:50**
Paraffin sections: The antibody can be used for labelling formalin-fixed, paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with trypsin, proteinase K or heat-induced epitope retrieval is required. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure.
Frozen sections and cell preparations: The antibody cannot be used for labelling frozen sections or cell smears owing to cross-reactivity with some cytokeratin-20-negative epithelia (e.g. renal tubules).Notes *Predicted MW = 46 kDa **Use 20 min heat-induced epitope retrieval and 30 min incubation at room temperature with the primary antibody. Storage Cytokeratin 20 (Ks 20.8) Monoclonal Antibody Store between 2-8°C. References Harnden P, Mahmood N, Southgate J. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 353:974-77, 1999.
Moll R. Cytokeratins as markers of differentiation in the diagnosis of epithelial tumors. In: Herrmann, Harris, editors. Subcellular biochemistry. Volume 31, New York: Plenum Press p205-62, 1998.
Savera AT, Torres FX, Linden MD, Bacchi CE, Gown AM, Zarbo RJ. Primary versus metastatic pulmonary adenocarcinoma. An immunohistochemical study using villin and cytokeratins 7 and 20. Appl Immunohistochem 4:86-94, 1996.
Wauters CCAP, Smedts F, Gerrits LGM, Bosman FT, Ramaekers FCS. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 26:852-55, 1995.
Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 140:427-47, 1992.
Moll R, Schiller DL, Franke WW. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J Cell Biol 111:567-80, 1990.
Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11-24, 1982.
Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Cytokeratin 20 (Ks 20.8) Monoclonal Antibody
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Cytokeratin 20 (Ks 20.8) Monoclonal Antibody
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