- 询价
- R&D
- ZK-0102213
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- Cytokeratin (OSCAR) Monoclonal Antibody, Purified (SIGNET)详细信息：
-
Description Monoclonal Antibody against Cytokeratin (OSCAR)
(Formerly Signet Catalog Nos: 465-01, 465-16 L1 Predilute, 465-26 L2 Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Clone OSCAR Form Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse IsoType Cytokeratin (OSCAR) Monoclonal Antibody, Purified (SIGNET)IgG2a Specificity Covance’s anti-cytokeratin antibody clone OSCAR demonstrates a broad spectrum of cytokeratin reactivity in immunoblotting. OSCAR is reactive with most epithelial types (in normal tissues) and with most carcinomas.
This antibody was developed using a crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
Concentrated Format:
SIG-3465-1000 [1 mL]
Prediluted Formats:
SIG-3465-16 [L1 6 mL]
SIG-3465-26 [L2 6 mL]
Both are ready-to-use with either a Biotin-based (USA Ultra Streptavidin Detection, SIG-32250) or Biotin-free (ACUITYAdvanced Polymer Detection, SIG-32902) detection systems.Uses This antibody is effective in immunoblotting and immunohistochemistry (IHC). Suggested Working Dilution Cytokeratin (OSCAR) Monoclonal Antibody, Purified (SIGNET)The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as citrate buffer retrieval solution (SIG-31932, SIG-31934).
Incubation: 20 minutes at room temperature
Notes Positive tissue (human): Skin, Liver, Bladder, Breast Storage Store between 2-8°C. References Bahrami A, Gown AM, Baird GS, Hicks MJ, Folpe AL. Aberrant expression of epithelial and neuroendocrine markers in alveolar rhabdomyosarcoma: a potentially serious diagnostic pitfall. Mod Pathol 21(7):795-806, 2024.
Gown, AM, Yaziji H, Barry TS, Hwang HC. OSCAR, A novel broad anti-cytokeratin monoclonal antibody optimized for diagnostic immunohistochemistry. US Canadian Acad Pathol Ann Meeting, 2003.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
- Cytokeratin (OSCAR) Monoclonal Antibody, Purified (SIGNET)
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Cytokeratin (OSCAR) Monoclonal Antibody, Purified (SIGNET)
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