- 询价
- R&D
- ZK-0102209
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- ACUITY Biotin Free Polymer Detection HRP System 150 Tests (SIGNET)详细信息：
-
Description ACUITY Biotin Free Polymer Detection HRP System, 150 Tests
(Formerly Signet Catalog No.: 2604)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form Detection Kit Specificity ACUITY Biotin Free Polymer Detection HRP System 150 Tests (SIGNET)Signets ACUITY Detection system is a sensitive biotin-free polymer detection kit that is compatible with antibodies raised in mouse, rat and rabbit. ACUITY contains a horseradish Peroxidase labeled polymer and special blocking solutions to eliminate possible nonspecific background staining.
Kit Contains:- Serum Block, 15 mL
- Special Block, 15 mL
- HRP Polymer Link, 15 mL
Suggested Working Dilution
Protocol:
1. Tissue preparation: Deparaffinize and rehydrate according to the established protocols.
2. Blocking step-1: Incubate with H202 at RT for 15 minutes. Rinse with PBS.
ACUITY Biotin Free Polymer Detection HRP System 150 Tests (SIGNET)3. Blocking step-2: Apply the Serum Block reagent for 15 minutes.
4. Primary antibody: Apply the primary antibody for 30 minutes.
5. Blocking step-3: Apply the Special Block reagent for 20 minutes then rinse with PBS (5min/2X).
6. Polymer Link: Apply the Polymer reagent for 30 minutes then rinse with PBS (5min/2X).
7. Chromogen step: Apply DAB or AEC for 3-5 minutes then rinse with DI water.
8. Counter stain & mount.Notes Discard unused DAB or AEC working solution at the end of the run. Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - ACUITY Biotin Free Polymer Detection HRP System 150 Tests (SIGNET)
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ACUITY Biotin Free Polymer Detection HRP System 150 Tests (SIGNET)
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