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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
来电咨询
- 克隆性:
多克隆
- 标记物:
见 说 明 书
- 宿主:
Goat,Rabbit,Mouse
- 适应物种:
人,大鼠,小鼠,兔
- 形态:
液态/粉状
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 保存条件:
-20℃保存
- ER81 Polyclonal Antibody详细信息:
-
Description Polyclonal Antibody against S100A4 Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone S100A4 Form Purified Antibody (in 0.1 M NaCl, 0.015 M sodium azide, pH 7.2) Host Rabbit Species Reactivity Mouse, Rat, Dog, Cow, Cat, Horse [Ab] ER81 Polyclonal Antibody0.72mg/mL Specificity The S100A4 protein belongs to the S100 subfamily, which has grown to be one of the largest subfamilies of the EF-hand Ca2+-binding proteins. The S100 proteins are small (10-12 kDa), acidic proteins sharing 25-65% sequence homology. A majority of the S100 genes cluster on human chromosome 1q21. The discovery of this cluster has provided the basis for a new nomenclature for S100 proteins encoded by genes in the 1q21 region. These proteins are now designated S100A1-S100A13. In immunoblotting analysis using recombinant S100 proteins (S100A1, S100A2, S100A4, S100A6 and S100B) only the corresponding antigen (S100A4) was recognized by the A 5114 antibody. No cross-reaction with the other S100 proteins was observed.
As demonstrated by immunocytochemistry, the antibody cross-reacts with the S100A4 equivalent protein in cat, cow, dog, horse, mouse and rat.
This antibody was raised against purified recombinant human S100A4 monomer expressed by E. coli.Uses ER81 Polyclonal AntibodyThis antibody is effective in immunoblotting, immunocytochemistry (ICC) and immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:100*
- IHC: 1:50 - 1:100**
Notes *Predicted MW = 10-12kDa
**This antibody has been used at a dilution range of 1:100-1:200 when applied on formalin-fixed, paraffin-embedded sections of human tonsil along with a 20 min heat-induced epitope retrieval, and 30 min primary antibody incubation at room temperature.Storage Store between 2-8°C. References Schäfer BW, Heizmann CW. The S100 family of EF-hand calcium-binding proteins: functions and pathology. Trends Biochem Sci 21:134-40, 1996.
Engelkamp D, Schäfer BW, Mattei MG, Erne P, Heizmann CV. Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E. Proc Natl Acad Sci 90:6547-51, 1993.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - ER81 Polyclonal Antibody
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zk-0312R-zky3 Rabbit Anti-Avidin/Cy3Cy3标记的兔抗亲和素
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zk-0312R-Bio Rabbit Anti-Avidin/Bio生物素标记的兔抗亲和素
zk-0312R-zkE-zkY5 Rabbit Anti-Avidin/PE-CY5PE-CY5标记的兔抗亲和素
zk-0312R-Azkzk Rabbit Anti-Avidin/APCAPC标记的兔抗亲和素
zk-0312R-AF350 Rabbit Anti-Avidin/Alexa Fluor 350Alexa Fluor 350标记的兔抗亲和素
zk-0312R-AF488 Rabbit Anti-Avidin/Alexa Fluor 488Alexa Fluor 488标记的兔抗亲和素
zk-0312R-AF555 Rabbit Anti-Avidin/Alexa Fluor 555Alexa Fluor 555标记的兔抗亲和素
zk-0312R-AF647 Rabbit Anti-Avidin/Alexa Fluor 647Alexa Fluor 647标记的兔抗亲和素
zk-0312R-zkE-zky5.5 Rabbit Anti-Avidin/PE-Cy5.5PE-Cy5.5标记的兔抗亲和素
zk-0312R-zkE-zky7 Rabbit Anti-Avidin/PE-Cy7PE-Cy7标记的兔抗亲和素
zk-0311R-zky3 Rabbit Anti-Biotin/Cy3Cy3标记的兔抗生物素抗体IgG
zk-0311R-zky5 Rabbit Anti-Biotin/Cy5Cy5标记的兔抗生物素抗体IgG
zk-0311R-zky5.5 Rabbit Anti-Biotin/Cy5.5Cy5.5标记的兔抗生物素抗体IgG
zk-0311R-zky7 Rabbit Anti-Biotin/Cy7Cy7标记的兔抗生物素抗体IgG
zk-0311R-zkE Rabbit Anti-Biotin/PEPE标记的兔抗生物素抗体IgG
zk-0311R-zkE-zky3 Rabbit Anti-Biotin/PE-Cy3PE-Cy3标记的兔抗生物素抗体IgG
zk-0311R-zkE-zkY5 Rabbit Anti-Biotin/PE-CY5PE-CY5标记的兔抗生物素抗体IgG
zk-0311R-Azkzk Rabbit Anti-Biotin/APCAPC标记的兔抗生物素抗体IgG
zk-0311R-AF350 Rabbit Anti-Biotin/Alexa Fluor 350Alexa Fluor 350标记的兔抗生物素抗体IgG
zk-0311R-AF488 Rabbit Anti-Biotin/Alexa Fluor 488Alexa Fluor 488标记的兔抗生物素抗体IgG
zk-0311R-AF555 Rabbit Anti-Biotin/Alexa Fluor 555Alexa Fluor 555标记的兔抗生物素抗体IgG
zk-0311R-AF647 Rabbit Anti-Biotin/Alexa Fluor 647Alexa Fluor 647标记的兔抗生物素抗体IgG
zk-0311R-zkE-zky5.5 Rabbit Anti-Biotin/PE-Cy5.5PE-Cy5.5标记的兔抗生物素抗体IgG
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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