- 询价
- R&D
- ZK-0101927
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- Keratin 1 Polyclonal Antibody, Purified详细信息：
-
Description Polyclonal Antibody against Keratin 1 (AF 87) Intended Use ** In Vitro Diagnostic (IVD) ** Form Affinity Purified Antibody (Stored in PBS + 0.03% Thimerosal) Host Rabbit Species Reactivity Human [Ab] Keratin 1 Polyclonal Antibody, Purified 1 mg/mL Specificity This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the human keratin 1 protein. It was subsequently purified using peptide affinity chromatography. Uses This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - WB: 1:1,000*
- IF: 1:1,000
- IHC: 1:500 with either biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250) or polymer based detection systems such as ACUITYAdvanced Biotin Free Polymer Detection (SIG-32902).
Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (SIG-31932, SIG-31934).
Incubation: 60 minutes at room temperature
Notes *Predicted MW = 67 kDa Storage Keratin 1 Polyclonal Antibody, Purified When stored at ≤20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first. References Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989. [WB, IF]
Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984. [WB, IF]Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Keratin 1 Polyclonal Antibody, Purified
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zk-0330R-Golzk Rabbit anti-mouse Kappa light chain/Gold胶体金标记的兔抗小鼠k链
zk-0330R-zky3 Rabbit anti-mouse Kappa light chain/Cy3Cy3标记的兔抗小鼠k链
zk-0330R-zky5 Rabbit anti-mouse Kappa light chain/Cy5Cy5标记的兔抗小鼠k链
zk-0330R-zky5.5 Rabbit anti-mouse Kappa light chain/Cy5.5Cy5.5标记的兔抗小鼠k链
zk-0330R-zky7 Rabbit anti-mouse Kappa light chain/Cy7Cy7标记的兔抗小鼠k链
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zk-0330R-AF555 Rabbit anti-mouse Kappa light chain/Alexa Fluor 555Alexa Fluor 555标记的兔抗小鼠k链
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Keratin 1 Polyclonal Antibody, Purified
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