- 询价
- R&D
- ZK-0101849
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- JSB1 Monoclonal Antibody (SIGNET)详细信息：
-
Description Monoclonal Antibody against JSB1
(Formerly Signet Catalog No: 8730-01)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone JSB-1 Form Tissue Culture Supernatant (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse Species Reactivity JSB1 Monoclonal Antibody (SIGNET)Human IsoType IgG1 Specificity JSB-1 identifies an independent, strongly conserved cytoplasmic epitope present on the mammalian MDR1 P-glycoprotein molecule. JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carbozklase (PC), an abundant 130kDa mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections. Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein expresssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
This antibody was raised against a multidrug-resistant Chinese hamster ovary cell line (CHrC5).Uses This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunocytochemistry (ICC), immunopurification (IP) and ELISA. Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 for Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues.
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (SIG-31910).
Incubation: 20-60 minutes at room temperature.
Notes Positive Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives.
Positive Tissue: Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretaory epithelium).Storage JSB1 Monoclonal Antibody (SIGNET) References Scheper RJ, et al. Monoclonal Antibody JSB-1 Detects a highly conserved epitope on the P-glycoprotein associated with multidrug-resistance. Int. J. Cancer 42:389-394, 1998.
Schinkel AH, et al. Characterization of the human MDR3 P- glycoprotein and its recognition by P-glycoprotein-specific monoclonal antibodies. Cancer Res. 51:2628-2635, 1991.
Tiirikainen MI, et al. Flow cytometric analysis of P-glycoprotein in normal and leukemic cells. Annals Hematol. 65:124-130, 1992.
Goasquen JE, et al. Expression of the multidrug resistance P-glycoprotein (P-170) in 59 cases of de novo acute lymphoblastic leukemia: prognostic implications. Blood 81:2394 - 2398, 1993.
Itsubo M, et al. Immunohistochemical Study of Expression and Cellular Localization of the multidrug resistance gene product P-glycoprotein in primary liver carcinoma.Cancer 73:298-303, 1994.
Klimecki WT, et al. P-glycoprotein expression and function in circulating blood cells From normal volunteers. Blood 83:2451- 2458, 1994.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 for Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
- JSB1 Monoclonal Antibody (SIGNET)
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JSB1 Monoclonal Antibody (SIGNET)
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