Stemgent® StainAlive™ DyLight™

Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody详细信息：
Product Overview

Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody was screened on live, unfixed mouse embryonic stem (ES) cells using immunocytochemistry (ICC). This antibody was selected as the best conjugated SSEA-1 antibody available for visualization of EC/ES/iPS cells, utilizing direct fluorescent staining and real-time imaging without fixation or killing the cells to direct visualization of the reprogramming process to iPS colonies. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide and is confirmed to be free of bacteria, fungi, mycoplasma and mouse virus. The live ES cell morphology, proliferation, and expression of pluripotency markers did not change after staining with this antibody. The MC-480 antibody reacts with mouse and human stage-specific embryonic antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis X carbohydrate structure. It is expressed on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated stem cells and a negative surface marker for human undifferentiated stem cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression. SSEA-1 is associated with cell adhesion, migration and differentiation.

Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 AntibodyProduct Specifications

Size / Quantity 50 µg (100 µl at 0.5 mg/ml)
Lot Number 1314
Immunogen Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Reactivity Mouse
Clone MC-480
Isotype Mouse IgM, κ
Formulation Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 1 EU/μg of the protein (< 0.1 ng/μg of the protein) as determined by the LAL assay. It is free from bacteria, fungi, mycoplasma, and mouse virus as tested.
Preparation The antibody was purified by affinity chromatography, and conjugated with DyLight™ 488 under optimal conditions. The solution is free of unconjugated dye and free antibody.
Storage and Handling Store at 4°C. Do not freeze. Light sensitive. This formulation contains no preservatives. Handle under aseptic conditions.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by immunocytochemistry (Figure 1)
Recommended Usage Dilute 1:100 for immunocytochemistry. For general protocols for performing viable cell immunocytochemistry directly on stem cells, see Technical Documents on the Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody product page at #09-0067.
Related Products Stemgent® DyLight™ 488 anti-SSEA-1 Antibody (Cat. # 09-0070)
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 Antibody (Cat. # 09-0069)
Notice to Purchaser Purchaser represents and warrants that it will use Stemgent® StainAlive™ Dylight™ 488 Mouse anti-SSEA-1 Antibody purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody
zk-4034R PDHA1丙 酮酸脱氢酶α1抗体
zk-4036R phospho-PDHA1(Ser293)磷酸化丙 酮酸脱氢酶α1抗体
zk-2739R PDPK13磷酸肌醇依赖性蛋白激酶1抗体
zk-3017R Phospho-PDPK1(Tyr373 + Tyr376)磷酸化3磷酸肌醇依赖性蛋白激酶1抗体
zk-2740R p53BP1p53结合蛋白1抗体
zk-3726R phospho-PKC delta (Tyr52)磷酸化蛋白激酶C亚性D型抗体
zk-3727R phospho-PKC delta (Thr505/507)磷酸化蛋白激酶C亚性D型抗体
zk-10399R PKC zeta蛋白激酶C zeta 抗体
zk-7340R PRG2嗜酸性粒细胞颗粒关键碱性蛋白抗体
zk-1664R Proinsulin胰岛素原抗体
zk-15501R PCSK4原蛋白转化酶4抗体
zk-4978R PMCA1细胞膜钙ATP酶1抗体
zk-4972R PCK1磷酸烯醇丙 酮酸羧激酶抗体
zk-4966R PAPOA多聚合酶α抗体
zk-4968R PAPOG多聚合酶g抗体
zk-11534R QRFP神经内分泌肽QRFP抗体
zk-6394R Quiescin Q6巯基氧化酶1抗体
zk-11668R QPRT/QAPRTase喹 啉酸磷酸核糖基转移酶抗体
zk-11579R QK1RNA结合蛋白QK1抗体
zk-11580R RAI3维甲酸诱导蛋白3抗体
zk-11581R RAPGEF2Rap鸟嘌呤核苷酸交换因子2抗体
zk-11582R RAPGEF5/RepacRap鸟嘌呤核苷酸交换因子(GEF)5抗体
zk-11583R RND2/Rho7Rho家族GTP酶2抗体
zk-11752R REEP1受体辅助蛋白1抗体
zk-11753R RNF135环指蛋白135抗体
zk-11669R RNF146环指蛋白146抗体
zk-11783R Rab5bRAS相关蛋白Rab5B抗体
zk-7828R RINT1胞膜间内质网调节蛋白1抗体
zk-7829R RMND1减数分裂同源蛋白1抗体
zk-7845R RASSF7RAS家族关联结构域蛋白7抗体
zk-6941R RNF74环指蛋白74抗体（重组激活基因1）
zk-6960R RAG2重组激活基因2蛋白抗体
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibodyzk-9481R REEP5受体表达蛋白5/息肉相关蛋白抗体
zk-11005R FAM64A染色体分离调节蛋白1抗体