- 询价
- R&D
- ZK-0101760
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)保存于运输说明：
- Store at room temperature
- 详细信息：
-
Description Retrieve-All Antigen Unmasking System 1: Universal (pH 8); 10x
(Formerly Signet Catalog No.: 1914)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)Antigen Unmasking Solution Specificity Retrieve-All is an antigen un-masking solution available in the following three pH formulas for use in heat induced un-masking procedures:
Retrieve-All 1 (Universal pH 8)
Retrieve-All 2 (Basic pH 10)
Retrieve-All 3 (Acidic pH 4.8)Suggested Working Dilution The optimal antigen unmasking condition is a function of temperature (90 to 100°C) and incubation time (5-30 minutes) and should be established by each investigator.
The Retreive-All working solution should be prepared by mixing 100 mL of 10x Retrieve-All into 900mL of water.
Protocol
1. Deparaffinize sections, rehydrate through graded alcohols, DI water, into PBS for 5 minutes.
2. Preheat Retrieve-All buffer to 92°C*.
Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)3. Place slides into the preheated Retrieve-All buffer.
4. Incubate for 10 minutes at 92°C.
5. Remove the container with slides and let it cool at room temperature for 5-10 minutes.
6. Rinse in distilled water or PBS.
7. Proceed with usual immunohistochemical staining.Notes *Concentrated buffers (100x) may show minimal precipitation after long term storage without any effect to the HIER. Storage Store at room temperature. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)
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Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)
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