- 询价
- R&D
- ZK-0101079
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- Keratin 14 Polyclonal Antibody, Purified保存于运输说明：
- Human, Mouse
- 详细信息：
-
Description Polyclonal Antibody against Keratin 14 [K14] (AF 64) Intended Use ** In Vitro Diagnostic (IVD) ** Form Affinity Purified Antibody (Stored in PBS + 0.03% Thimerosal) Host Rabbit Species Reactivity Human, Mouse [Ab] 1 mg/mL Specificity Keratin 14 Polyclonal Antibody, Purified This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 14 protein. It was subsequently purified using peptide affinity chromatography. Uses This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - WB: 1:1,000*
- IF: 1,000
- IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250) or polymer based detection systems such as ACUITYAdvanced Biotin Free Polymer Detection (SIG-32902).
Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (SIG-31932, SIG-31934).
Incubation: 60 minutes at room temperature
Notes *Predicted MW = 55 kDa Storage When stored at ≤20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first. References Keratin 14 Polyclonal Antibody, Purified Welm AL, Kim S, Welm BE, Bishop JM. MET and MYC cooperate in mammary tumorigenesis.Proc Natl Acad Sci USA 102(12):4324-9, 2005. [IHC]
Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001. [IF]
Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.[WB, IF]
Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Keratin 14 Polyclonal Antibody, Purified
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Keratin 14 Polyclonal Antibody, Purified
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