IFN a 2b Human, Yeast

IFN a 2b Human, Yeast

• IFN a 2b Human, Yeast
• Interferon-Alpha 2b Human Recombinant, Yeast
• 10µg ($50)
  50µg ($130)
  1mg ($1400)

   

CATALOGUE NUMBER

CYT-1460

SYNONYMS

Interferon alpha 2b, IFNA, INFA2, IFN-? 2b, MGC125764, MGC125765.

INTRODUCTION

IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.

DESCRIPTION

Interferon-alpha 2b Human Recombinant produced in yeast is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of approximately 19 kDa.
The Interferon-alpha 2b gene was obtained from human leukocytes.
The IFN-a 2b is purified by proprietary chromatographic techniques.

SOURCE

Saccharomyces cerevisiae.

PHYSICAL APPEARANCE

Sterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH-7.4.

SOLUBILITY

It is recommended to reconstitute the lyophilized glycosilated IFN alpha 2b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

STABILITY

Lyophilized glycosilated IFN-a 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

PURITY

Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

AMINO ACID SEQUENCE

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln.

BIOLOGICAL ACTIVITY

The specific activity as determined in a viral resistance assay was found to be no less than 300,000,000IU/mg.

USAGE

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.