IFN b 1b Human

IFN b 1b Human

• IFN b 1b Human
• Interferon-Beta 1b Human Recombinant
• 2µg ($50)
  10µg ($130)
  1mg ($2700)

   

CATALOGUE NUMBER

CYT-1234

SYNONYMS

Leukocyte interferon, B cell interferon, Type I interferon, IFNB1, IFB, IFF, IFNB, IFN-b 1b, MGC96956.

INTRODUCTION

Interferon-beta 1b has antiviral, antibacterial and anticancer activities.

DESCRIPTION

Interferon beta 1b Human Recombinant produced in E.Coli is a single, non-glycosylated mutein (variant form) of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton.
The IFN-beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17. 
IFN beta-1b is purified by proprietary chromatographic techniques.

SOURCE

Escherichia Coli.

PHYSICAL APPEARANCE

Sterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

Lyophilized from 1mg/ml solution containing 50mg Human Albumin & 50mg dextrose.

SOLUBILITY

It is recommended to reconstitute the lyophilized Interferon beta-1b in sterile 18M-cm H2O at a concentration of 0.25mg/ml, which can then be further diluted to other aqueous solutions.

STABILITY

Lyophilized Interferon-beta 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFNB 1b should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

PURITY

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC. 
(b) Analysis by SDS-PAGE.

AMINO ACID SEQUENCE

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.

BIOLOGICAL ACTIVITY

The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 32 x 10⁶ IU/mg.

PROTEIN CONTENT

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard.

USAGE

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.