Anti-Aquaporin 3

Anti-Aquaporin 3

AQP3, GLIP
Cat #: AQP-003
Sizes: 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IFC, IH, IP, WB
May also work in: IC
Reactivity: H, M, R

Anti-Aquaporin 3Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Anti-Aquaporin 3 (#AQP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunohistochemistry applications. It has been designed to recognize Aquaporin 3 from rat, human, and mouse samples.

For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.

• Applications
• Specifications
• Scientific Background
• Citations
• Related Products

Western blot

Western blot analysis of rat kidney membranes:
1. Anti-Aquaporin 3 antibody (#AQP-003), (1:200).
2. Anti-Aquaporin 3 antibody, preincubated with the control peptide antigen.
Immunoprecipitation
Mouse epidermal keratinocytes (1 μg per ml) (see Zheng, X. and Bollinger Bollag, W. (2003) J. Invest. Dermatol. 121, 1487.).
Immunohistochemistry

Expression of Aquaporin 3 in rat colon
Immunohistochemical staining of paraffin embedded longitudinal section of rat colon using Anti-Aquaporin 3 antibody (#AQP-003), (brown). Aquaporin 3 is detected in absorptive cells that are present in the luminal epithelium and in the superior third of the intestinal glands (arrows). Hematoxilin is used as the counterstain. mucosa (M), submucosa (SM) and muscularis externa (ME).
Indirect flow cytometry
Human airway epithelial cell (see Avril-Delplanque, A. et al. (2005) Stem Cells 23, 992.).
The control antigen is not suitable for this application.

Immunogen
Peptide (C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3 (Accession P47862).  Intracellular, C-terminus.

HomologyMouse - 17/18 amino acid residues identical; sheep, human - 15/18 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted powder contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.4 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 μg peptide per 1 μg antibody.
Scientific background

Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).

The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7, AQP-9 and AQP-10.¹
Little is known about the function of the two newest members, AQP-11 and AQP-12.  

The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.¹

AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis.² Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.³

References

1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
2. Hara, M. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 7360.
3. Hara-Chikuma, M. and Verkman, A.S. (2024) Mol. Cell. Biol. 28, 326.