pAS2

Restriction digests of the clone give the following sizes (kb): BamHI–8.6; SalI–8.6; SmaI–8.6. (ATCC staff) Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin) epitope tag in frame with the activation domain. [1] The order of the major features in this plasmid is: ADC1 (ADH) promoter -> – GAL4 DNA binding domain – HA – NdeI/MCS/SalI – ADC1 terminator – pMB1 ori – ampR – 2 micron ori – TRP1 -> – f1 ori – <- CYH2. [2] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Elledge S.J. Hosts: E.coli, yeast, Saccharomyces cerevisiae.